K-ACETAK，***【AK法】检测***盒，ACETIC ACID(ACETATE KINASE FORMAT) assay Kit

产品识别号：K-ACETAK

产品品名：***【AK法】检测***盒

英文品名：ACETIC ACID(ACETATE KINASE FORMAT) assay Kit

规格型号：550次（170.5 ml）

Analyser format for the specific assay of acetic acid (acetate) inbeverages and food products. On calibration, the prepared reagent islinear to > 28 micrograms of acetic acid per mL of assay solution.

INTRODUCTION:

The most widely used method for enzymatic quantification of acetic

acid is that employing acetyl-coenzyme A synthetase (ACS). However,

this method is based on the use of an indicator reaction catalysed by

L-malate dehydrogenase that is in permanent equilibrium, and therefore

a non-stoichiometric increase in absorbance is observed from the

acetate present in the sample. Thus, a slightly non-linear resp***e

to increasing acetate concentration is observed upon calibration,

resulting in poor R2 values. In addition (depending on the supplier)

reagents prepared for auto-analyser applicati*** can h***e very limited

on-machine stability, due to rapidly increasing blank absorbance values.

To overcome these issues, Megazyme developed this alternative acetic

acid kit, based on the enzyme acetate kinase (AK; see equati*** 1-3

below), especially for the auto-analyser user. This reagent has improved

on-machine stability, gives excellent linear calibration curves, and results

in a stoichiometric change in absorbance due to the acetic acid present

in the sample.

KITS:

Kits suitable for performing a minimum of 550 assays in auto-analyser

format are ***ailable from Megazyme.

The kits contain the full assay method plus:

Bottle 1: Buffer (11 mL, pH 7.4) plus sodium azide (0.02% w/v ) as a

preservative. Stable for > 2 years at 4°C.

Bottle 2: NADH plus ATP, PEP and PVP.

Stable for > 2 years at either 4°C or -20°C.

Bottle 3: Acetate kinase plus pyruvate kinase and D-lactate

dehydrogenase suspension, 4.1 mL.

Stable for > 2 years at 4°C.

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