PH荧光探针BCECFBECEF被广泛地用于细胞内pH的测定。它的pKa值为6.97，接近生理PH值，它可以灵敏监测细胞内溶液PH的变化。细胞内PH值较低时BECEF的荧光很弱，随着PH的升高，荧光增强幅度很大。505nm和439nm通常是非常有用的用于比率测量的激发波长，一般会将490nm和450nm的滤光片加在激发光源的前面，比率法测量一般是在530nm的相对荧光强度下进行的。BCECFacid是膜不通透型的，因此必须用微***方法直接进入细胞内。BCECF-AM是乙酰甲酯化的BCECF，它通过孵育就能进入细胞。订购信息：货号产品名称规格51009BCECF,AMester,1mg/mLinanhydrousDMSO1ml51010BCECFfreeacid(2',7'-bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein)1mg51011BCECF,AMester10x100ug51011-1BCECF,AMester20x50ug51012BCECF,AMester1mg510135-(and-6)-Carboxyfluorescein(5-(and-6)-FAM,mixedisomer)100mg510145(6)-CFDA(5-(and-6)-Carboxyfluoresceindiacetate)100mg510155-(and-6)-carboxy-2',7'-dichlorofluorescein200mg510165-(&6)-carboxy-2',7'-dichlorofluoresceindiacetate100mg510175-carboxy-2',7'-dichlorofluorescein(singleisomer)100mg510185-CFDA(5-Carboxyfluoresceindiacetate,singleisomer)100mg510195-Carboxyfluorescein(5-FAM,singleisomer)100mg510216-CFDA(6-Carboxyfluoresceindiacetate,singleisomer)100mg51022Flubida-25mg510235-carboxy-2',7'-dichlorosulfonefluorescein10mg51024Flubi-25mg900405-(and-6)-Carboxy-2',7'-dichlorofluoresceindiacetate,succinimidylester25mg900415(6)-CFDA,SE(5-(and-6)-Carboxyfluoresceindiacetate,succinimidylester)25mg)