氨基反应的生物素交联剂，NHS-Biotin，NHS-LC-LC-Biotin，SulfoNHS-Biotin西安瑞禧生物科技有限公司提供与氨基反应的生物素交联剂，介绍如下：N-hydroxysuccinimidylesters(NHS)ofbiotinsareexcellentreagentsforbiotinylatingbiomoleculessuchasproteinsandamine-modifiednucleicacids.NHS-Biotin(Order-No.B001N)isthesimplestbiotinylatingreagent***ailable.Whenthetargetaminesarelessreactive(i.e.secondaryamines)afluorophenolactiveesterlikePFP-Biotin(Order-No.B010F)ispreferred.Bothreagentsareinsolubleinaqueousmedia,andmustbefirstdissolvedinnon-proticorganicsolventssuchas***orDMSO,thenaliquotintotheaqueousreactionmixture.Becausethesecompoundsarepronetohydrolysisbywater,itisrecommendedtopreparefreshstocksoluti***toachievethebestyieldatafinalconcentrationoforganicsolventnotabove10%.Similarproceduresareemployedfor:NHS-LC-BiotinNHS-LC-LC-BiotinWater-solubleanalogsofNHSesterbiotinsarealso***ailable,like:SulfoNHS-Biotin(Order-No.B101N)SulfoNHS-LC-Biotin(Order-No.B102N)SulfoNHS-LC-LC-Biotin(Order-No.B103N)TheseactiveesterscontainanegativelychargedsulfonateintheirNHSringstructure.Theycanbeaddeddirectlytoaqueousreactionmedia,withouttheneedoforganicsolvents.Concentratedstocksoluti***maybeprepared,butmustusedimmediatelyastheyhydrolyzequickly.Anotherwater-solubleSulfoNHSesterlikeSulfoNHS-SS-Biotin(Order-No.B104N)containsaspacerarmwhichcanbecle***edundermildconditi***.Reductionofthedisulfidebondwith50mMdithiothreitolreleasesthebiotinylatedcomponentfromthe***idineorstrept***idinelabeleddetectionreagents.Cyanagendevelopedanewclassofwater-solubleBiotinNHSestersbasedonhydrophilicpolyethyleneoxide(PEO)spacerarmslike:NHS-PEO3-Biotin(Order-No.B040N)NHS-PEO4-Biotin(Order-No.B041N)NHS-PEO5-Biotin(Order-No.B042N)详细的生物素交联***的产品信息如下：NHS-BiotinMW:341.38NHS-LC-BiotinMW:454.54NHS-LC-LC-BiotinMW:567.70NHS-PEO3-BiotinMW:500.58NHS-PEO4-BiotinMW:544.63NHS-PEO5-BiotinMW:588.68SulfoNHS-BiotinMW:443.43SulfoNHS-LC-BiotinMW：556.60SulfoNHS-LC-LC-BiotinMW：669.74SulfoNHS-SS-BiotinMW：606.70PFP-BiotinMW：410.36订购联系信息：西安瑞禧生物科技有限公司电话：029-8635488513309186942***:31952518e-mail:sales@网站：)