产品编号产品名称CAS号产品包装1128905-溴-4-氯-3-吲哚-β-D-吡喃半乳糖苷（X-β-Gal，X-Gal）7240-90-61g023-150415-溴-4-氯-3-吲哚-β-D-吡喃半乳糖苷（X-β-Gal，X-Gal）7240-90-6100mg029-150435-溴-4-氯-3-吲哚-β-D-吡喃半乳糖苷（X-β-Gal，X-Gal）7240-90-61g024-167915-溴-4-氯-3-吲哚-α-D-吡喃半乳糖苷（X-α-Gal）107021-38-525mg020-167935-溴-4-氯-3-吲哚-α-D-吡喃半乳糖苷（X-α-Gal）107021-38-5100mg148941Isopropyl-β-D(-)-thiogalactopyranoside（IPTG）367-93-1100mg090-05141Isopropyl-β-D(-)-thiogalactopyranoside（IPTG）367-93-1100mg096-05143Isopropyl-β-D(-)-thiogalactopyranoside（IPTG）367-93-11g094-05144Isopropyl-β-D(-)-thiogalactopyranoside（IPTG）367-93-110g090-05146Isopropyl-β-D(-)-thiogalactopyranoside（IPTG）367-93-1100g025-153615-Bromo-4-chloro-3-indolyl-β-D-glucuronideCyclohexylammoniumSalt（X-Gluc）114162-64-010mg021-153635-Bromo-4-chloro-3-indolyl-β-D-glucuronideCyclohexylammoniumSalt（X-Gluc）114162-64-0100mg更多相关产品，欢迎咨询：上海西宝生物科技有限公司电话：021-50272975/76/77/78杨露13512172575QQ:1916572322分机6221俞婵13817370083QQ:2457655304分机6512杨树彦13788987962QQ:1961275959分机6236传真：021-50272982E-mail:trade@lifesci9@MSN:lifesci9@Http://地址：上海市张江高科技园区毕升路299弄11#502一、X-GalX-Gal也称5-Bromo-4-chloro-3-indolylβ-D-galactopyranoside或5-Bromo-4-chloro-3-indolylβ-D-galactoside，中文名为5-溴-4-氯-3-吲哚-β-D-半乳糖苷。分子式为C14H15BrClNO6，分子量为408.63，CASNumber7240-90-6，纯度>99%。进口分装。本产品为白色至浅黄色粉末。X-Gal是β-半乳糖苷酶(β-galactosidase)的显色底物，在β-半乳糖苷酶的催化下会产生蓝色产物。常用于β-半乳糖苷酶的原位染色检测以及蓝白斑筛选。溶于DMSO，溶解度可达20mg/ml。也溶于DMF。X-gal(alsoabbreviatedBCIGforbromo-chloro-indolyl-galactopyranoside)isanorganiccompoundconsistingofgalactoselinkedtoasubstitutedindole.Itisveryheavilyusedinmolecularbiology.UsesCloningIngenecloning,X-galisusedtoindicatewhetheracellexpressestheβ-galactosidaseenzyme,whichisencodedbythelacZgene,inatechniquecalledblue/whitescreening.X-galiscleavedbyβ-galactosidaseyieldinggalactoseand5-bromo-4-chloro-3-hydroxyindole.Thelatteristhenoxidizedinto5,5'-dibromo-4,4'-dichloro-indigo,aninsolubleblueproduct.Thus,ifX-galandaninducerofβ-galactosidase(usuallyIPTG)iscontainedwithinanagarmediumonacultureplate,colonieswhichhaveafunctionallacZgenecaneasilybedistinguished.Whenthetechniqueofcloningplasmidvectorgeneswithinbacterialcellsisoptimal,X-galisusedtovisuallylocateyeastorE.colicoloniesthathavebeentransformedbythedesiredplasmidvectorinablue-whitescreen.E.colibacteria,whichcannotproducetheenzymeβ-galactosidase(codedbylacZgeneofthelacoperon),aretransformedbyabsorbingtheplasmidvectors,whichcontainaninsert,inthelacZopenreadingframe.Afterthetransformationprocess,thebacteriaisspreadonnutrientagarplates,whichmostlycontainantibioticsaswell.Mostcommerciallyavailablevectorscontainanantibiotic-resistantgene.Successfullytransformedbacteriahasatruncatedβ-galactosidasegene,causingwhitecoloniesontheplate.Bacteriatransformedbyemptyvectors,whichdonotcontainaninsertinthelacZopenreadingframe,arenowabletoproducetheenzymeβ-galactosidasewhichcanthencleavetheX-galpresentwithinthenutrientagar,resultinginabluecolony.Bacteriacoloniesthatgrowfrombacteriathatwerenottransformeddonotcontainthisantibiotic-resistance,andthus,die.Theplasmidvectorscanalsobecodedtodisruptadifferentbacteria'sabilitytoproduceβ-galactosidase,causingthedesiredbacteriacoloniestogrowtobewhiteandnon-transformedcoloniestogrowtobeblue.Thisisthecasewithmanycommerciallyavailablecloningvectors,suchasPromega'spGem-TVectors,whichcarrylacZα,atruncatedformofβ-galactosidase,andrequirespecificE.colihostsastrain(suchasDH5α)toachieveα-complementation.ReporterThelacZgenemaybeusedasareporterincombinationwithgrowthmediacontainingX-gal.Intwo-hybridanalysisforexample,itisnecessarytodistinguishbetweenthoseyeastorbacteriainwhichthereisasuccessfulinteraction,leadingtothebindingofanactivationdomaintoapromoter,andthoseinwhichthereisnot.IfthepromoterislinkedtoalacZgene,theproductionofβ-galactosidasewillbeindicatedbytheproductionofbluepigmentbycoloniesthathostasuccessfulinteraction.[1]Duetoitsmanualnature,thistechniqueislimitedtosituationsinwhichthenumberofcoloniesthatmustbedistinguishedislessthanaround106.[1]ThesuccessfulcleavageofX-galalsocreatesanoticeablyfoulodorduetothevolatilizationofindole.WatertestingInadditiontouseinmolecularbiology,X-GalisusedtodetermineE.coliandcoliformcontentindrinkingwatersamples.二、IPTG中文品名:异丙基-β-D-硫代吡喃半乳糖苷商品名称:Isopropylβ-D-1-Thiogalactopyranoside英文简称:IPTG产品别名:Isopropylβ-D-Thiogalactoside通用CAS:367-93-1产品纯度:Sigma≥99%,Merck≥98%，INALCO≥99%产品性状:白色或白色粉末,溶于水后呈清亮无色液体分子式:C9H18O5S分子量:238.30保存温度:2-8°C冷藏保存,配制好后保存于-20°C，室温可放置一个月主要作用：异丙基硫代半乳糖苷(IPTG)是一种作用极强的诱导剂,不被细菌代谢而十分稳定,因此被实验室广泛应用IPTG是β–半乳糖苷酶的活性诱导物质。基于这个特性，当pUC系列的载体DNA（或其他带有lacZ基因载体DNA）以lacZ缺失细胞为宿主进行转化时、或用M13噬菌体的载体DNA进行转染时，如果在平板培养基中加入X–Gal和IPTG，由于β–半乳糖苷酶的α–互补性，可以根据是否呈现白色菌落（或噬菌斑）而方便地挑选出基因重组体。此外，它还可以作为具有lac或tac等启动子的表达载体的表达诱导物使用。Isopropylβ-D-1-thiogalactopyranoside,abbreviatedIPTG,isamolecularbiologyreagent.Thiscompoundisusedasamolecularmimicofallolactose,alactosemetabolitethattriggerstranscriptionofthelacoperon.Unlikeallolactose,thesulfur(S)atomcreatesachemicalbondwhichisnon-hydrolyzablebythecell,preventingthecellfrom"eatingup"ordegradingtheinductant;thereforetheIPTGconcentrationremainsconstant.Forinduction,asterile1MsolutionofIPTGistypicallyaddedby1:1000dilutionintoalogarithmicallygrowingbacterialculture.Higherconcentrationscanbeused.IPTGinducesthetranscriptionofthegenecodingforbeta-galactosidase,ahydrolaseenzymethatcatalyzesthehydrolysisofβ-galactosidesintomonosaccharides.OneadvantageofIPTGforinvivostudiesisthatsinceitcannotbemetabolizedbyE.coliitsconcentrationremainsconstantandtherateofexpressionoflacp/o-controlledgenes,isnotavariableintheexperiment.IPTGintakeisindependentontheactionoflactosepermease,sinceothertransportpathwaysarealsoinvolved.Incloningexperiments,coloniesthathavebeentransformedwiththerecombinantplasmidratherthananon-recombinantneedtobeidentified.X-galisasubstancethatcanbemetabolisedbybeta-galactosidasetoproduceablueproduct.Thuscellsexpressingbeta-galactosidasegrowninthepresenceofX-galandIPTG(toinducetheexpression)willturnblue.WhereaDNAfragmenthasbeeninsertedintotheLacZ(oneofthegenesforbeta-galactosidase)therewillbenoactionuponX-galandthecellswillnotturnblue,thusidentifyingthecellsthatcarryrecombinantplasmidratherthannon-recombinantplasmid.Manyregulatoryelementsofthelacoperonareusedininduciblerecombinantproteinsystems;IPTGisaneffectiveinducerintheconcentrationrangeof100μMto1.5mM.三、X-α-GalCAS:107021-38-5英明；5-Bromo-4-chloro-3-indoxyl-α-D-galactopyranoside;X-α-D-Galactoside分子式：C14H15BrCNO6分子量：408.64g/mol报告基因Mel表达后，在含X-α-Gal的平板上显蓝色溶解性：清澈溶液（2%inDMF）储存：4℃避光避潮；长期存放-20℃用途：X-α-半乳糖苷酶的作用底物，用于筛选含X-α-半乳糖苷酶基因的阳性酵母或细菌菌株。在组织化学中用于酶活性的检测。贮液：A)涂布于预制平板：溶解24mgX-α-gal于6mLDMF中得到终浓度为4mg/mL的溶液。B)直接加入琼脂中：溶解60mgX-α-gal于3mLDMF中得到终浓度为20mg/mL的溶液。参考用法：A)涂布于预制平板1.涂布200ul(15cm)或者100ul（10cm）X-α-gal贮液于预制平板上。2.放于37oC培养箱至液体被吸收（约4小时）。3.将转化细菌或酵母涂于平板上并于合适温度培养至蓝色菌落出现。B)直接加入琼脂中1.将已灭菌琼脂培养基冷却至55-50oC。2.在已冷却的培养基中加入20mg/mlX-α-Gal贮液，混匀，快速倒平板。四、X-glucCAS：18656-96-7分子式：C14H13BrClNO7.C6H13N分子量：521.8外观：白色粉末溶解性：清澈溶液（2％inDMF）用途：检测大肠杆菌中uidA基因（β-葡萄糖苷酶基因β-glucuronidase,GUS）的底物。95%的大肠杆菌具有β-葡萄糖苷酶基因，用这种发色底物的培养基可以检测以及定量食品样本如肉、奶制品以及贝类中的大肠杆菌数量，以及在临床上检测尿路感染。国际上普遍采用该产品取代传统方法以精确检测饮用水中的大肠杆菌数量，该方法大大降低了假阳性和假阴性。该产品也用于快速检测植物中GUS基因融合标记。β-葡糖醛酸酶（β-glucuronidase(GUS)）基因检测的显色底物。更多相关产品，欢迎咨询：上海西宝生物科技有限公司电话：021-50272975/76/77/78杨露13512172575QQ:1916572322分机6221俞婵13817370083QQ:2457655304分机6512杨树彦13788987962QQ:1961275959分机6236传真：021-50272982E-mail:trade@lifesci9@MSN:lifesci9@Http://地址：上海市张江高科技园区毕升路299弄11#502)