AmershamHybond-N+带正电荷尼龙膜0.45um30cmx3mRPN303B带正电荷的尼龙膜与核酸高达600μg/cm2的结合能力。建议使用***性和非***性的化学发光法和chemifluorescence检测系统。Amersham公司Hybond™-N+产品在传统的Southern杂交有着出色的灵敏度。核酸样品可能是固定的，通过紫外线照射或烘烤，虽然紫外线固定是***好的***大的重复性。HybondN+是不适合碱印迹和固定。AmershamHybond™-N+Positivelychargednylonmembranewithabindingcapacityfornucleicacidsupto600µg/cm2¾recommendedforusewithradioactiveornonradioactivechemiluminescenceandchemifluorescencedetectionsystems.Recommendedforallhighdensitymembranearrays.TechnicalInformationAmershamHybond™-N+yieldsexcellentsensitivityinconventionalSouthernblotting.NucleicacidsamplesmaybefixedbyUVexposureorbaking,althoughUVfixationisbestformaximumreproducibility.Hybond-N+isnotsuitableforAlkaliblottingandfixation.Multiplereprobingispossiblewithoutlossofmembraneintegrity.QCtestedingenomicSouthernblotsusingGEHealthcareproducts.Specialsizes***ailableonrequest.HumangenomicDNA(1,0.5,0.125,0.06and0.03µgloadings)digestedwithHindIIIandtransferredtoHybond-N+.HybridizatonwithA.N-ras,B.p53,andC.bcl-2labeledDNAprobesanddetectedusingGeneImages™random-primelabelingwithCDP-Star™detection.Membranesshouldbestoredatroomtemperatureinacleandryatmosphereawayfromexcessiveheat,light,andnoxiousfumes.Topreventcontamination,handlemembranesusingglovesorblunt-endedforceps.Hybond-N+isstableforupto3years.Membranesshouldbekeptinthebagsinwhichtheyarereceived.Performanceisc***istentwhenstoredundertherecommendedconditi***.TECHNICALSPECIFICATI***Poresize0.45µmNucleicacidsizerequired>50bp)