***与转化
RossetaCompententCell;Rosseta感受态细胞BL21(DE3)pLysSCompententCell;BL21(DE3)pLysS感受态细胞BL21(DE3)CompententCell;BL21(DE3)感受态细胞DH5αCompententCell;DH5α感受态细胞***0CompententCell;***0感受态细胞ColonyFastPCRKit;菌落PCR***盒T4DNALigase;T4连接酶A-TailingKit;加A***盒pUC-TsimpleCloningKit;pUC-TsimpleTA******盒Uracil-DNAGlycosylase;尿***DNA糖基化酶(UNG酶))