磷脂***盒PhospholipidsC(reagentkit)-CODDAOSmethodSummaryandexplanationofthetest:TheWakophopholipidsCisanenzymaticcolorimetricmethodusingN-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline(DAOS).Thisisreagenthashighspecificityandisnotinterferedwithascorbicacidandbilirubininserum.Principleofthemethod:Asshowninthechemicalreacti***below,Phospholipidsinserum(lecithin,lysolecithinandsphingomyelin)arehydrolyzedtofreecholinebyphospholipaseD.Theliberatedcholineissubsequentlyoxidizedtobetainebycholineoxidasewiththesimultaneousproductionofhydrogenperoxide.Thehydrogenperoxidewhichisproducedquantitatively,oxidativelycouples4-aminoantipyrineandDAOStoyieldachromogenwithmaximumabsorptionatλ=600nmReagents(For120tests)(1)BufferGood'sbuffer(pH7.5)50mmol/L8x50mL(2)ColorReagent(whenrec***tituted)8xfor50mLPhospholipaseD0.47U/mLCholineOxidase2.0U/mLPeroxidase4.2U/mLDAOS0.77mmol/L4-Aminoantipyrine0.24mmol/LAscorbicacidoxidase3.9U/mL(3)StandardSolution2x10mL(Correspondingto300mg/dLofphospholipidscontents)CholineChloride54mg/dLWarningsandPrecauti***ForInVitroDiagnosticUseNottobeusedinternallyinhumansoranimalsDonotmixorusethereagentsfromonetestunitwiththoseofanothertestunitwhichhasadifferentlotnumber.Donotusereagentspattheexpirationdatestatedoneachreagentcontainerlabel.SpecimenCollectionSerumAscorbicacid.Bilirubinandhemolysisdonotaffectthephospholipidsmeasurement.TheWakomethodmeasuresthe3kindsofphospholipidsinserum(lectithin,lysolecithinandsphingomyelin)butdoesnotmeasurecephalin.MaterialsrequiredbutnotsuppliedTesttubesPipettesWaterbath,capableofmaintaining37degreeCSpectrophotometerorColorimeter,capableofmeasuringabsorbanceat600nm.PreparationofreagentCOLORREAGENTSOLUTIONDissolvethecontentsofonevial(for50mL)ofColorReagentinonebottle(50mL)ofBuffer.Thissolutionisstablefor1weekat2-10degreeC.ProcedureSample(S)Standard(Std)Blank(Bl)PipetteintoatesttubeSampleSample0.02mLStandard0.02mL-*1COLORREAGENTSOLUTION3.0mL3.0mL3.0mLMixincubatefor5minutesat37degreeCandmeasuretheabsorbanceofSandStdagainstBlat600nm.Absorbance(600nm)ASAStdBlank*1:Theomissionof0.02mLofwaterdoesnotsignificantlyaffecttheabsorbancemeasured.*2:Whenmeasurewithtwow***elength:λ1/λ2=600/700nm.CAUTIONThecolorreactionfinishesinabout3minutesandthe5minutesofincubationat37degreeCisenoughforthereaction.Theextendedincubationwillcausethecolordeterioration.Please***oidkeepingthetesttubewarmlongerthan15minutes.Thecolorisstablefor1hourinroomtemperature.Results(Calculation)AS:AbsorbanceofsamplePhospholipids(mg/dL)=(AS/AStd)x300AStd:AbsorbanceofstandardLimitati***oftheprocedureThecalibrationcurveislinearupto1000mg/dLofphospholipidscontent.ExpectedValuesSerum:145-257mg/dLSinceexpectedvaluesareaffectedbyage,sex,diet,geographicallocationandotherfactors,eachlaboratoryshouldestablishitownexpectedvaluesforthisprocedure.AdditionalrecommendedproductsCodeNo.410-00101ControlSerumI(Normal)10xfor5mLCodeNo.416-00201ControlSerumII(Abnormal)10xfor5mLReferenceTakayamaM.,ItohS.,NagasakiT.andTanimizuI.:Clin.Chim.Acta.79,93-98(1977)++++++++++++++++++++++++++++++++++++++如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com在线***：***：1069267096MSN:liyuqiu1983@cindylee-1981@总机：010-60206266传真：010-60206773www.express-cn.com)