Ethynylestradiol(EE2)ELISAKit炔雌醇（EE2）***盒EE2ELISAKitEE2monoclonalantibodybindsexclusivelywithEE2anddoesnotshowcross-reactionwithotherhormonesorchemicalswithsimilarstructures.Thequantitativeanalysisrangesfrom0.05μg/L-3μg/L(ppb),sensitiveenoughtodetectEE2inaspecimenfromriverwaterorblood,etc.Witheaseofhandling,thetotaltimeformeasurementisonly2.5hours.TheELISAmeasurementishighlyreproducible;thecoefficientofvariation(CV)isgenerallyunder10%.Thekit,a96-wellmicroplateformat,enablessimultaneousmeasurementofmultiplesamplesatmorereasonablecost.EE2Ethynylestradiol(EE2),oneofmainingredientsforcontraceptivepill,issuspectedasoneoftheendocrinedisruptingchemicals.WhileGC-MSorLC-MS/MSaregenerallyemployedmethodforquantitativeanalysis,theyrequireexpensiveinstrumentationaswellascomplexandtime-c***umingextractionprocesswithhazardousorganicsolvent.ThistestkitdetectsEE2inenvironmentorinvitroatapptlevel(ng/L)withtheaidofsolidphaseextraction.EE2StandardCurveSamplescontainingEthynylestradiol(EE2)withinthedynamicrange(0.05μg/L-3μg/L)canbedirectlyappliedtoassayafterfiltration.SampleswithEthynylestradiolcontentbelowtherangemustbeconcentratedwithsolidphaseextractionpriortotheensuingsession.Coefficientofvariation(CV)isgenerallyunder10%throughoutthedynamicrange.Pretreatment(SimplifiedSolidPhaseExtraction)AlsoseehereAdjustthepHofthesampleto5.0with1MAceticacid.PoursampleliquidthroughaC18solid-phasecartridge/diskandeluteEE2with***ane.Drythesamplewithnitrogengas,thenadjustMeOHconcentrationat10%formeasurement.Thespecificproceduremayvaryinsamplequality.Cross-reactivityPatternCompoundReactivity(%)Ethynylestradiol(EE2)100.0Estrone(E1)<0.22-methoxyE1<0.217β-Estradiol(E2)<0.216-keto-E2<0.2E2-17-glucuronide<0.2E2-3-glucuronide<0.2E2-3-sulfate-17-glucuronide<0.2Estriol(E3)<0.216-epi-E3<0.2E3-16-glucuronide<0.2KitFormatKitFormatCommentMicroplate1plate(96wells)&reagentsNeedsamicroplatereader(450nm)Formultipleandsimultaneousmeasurement如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com在线***：***：1069267096MSN：liyuqiu1983@cindylee-1981@联系人：杨先生总机：010-60206266传真：010-60206773www.express-cn.com)