Estrone(E1)ELISAKit雌***酮***盒E1ELISAKitE1monoclonalantibodybindsexclusivelywithE1anddoesnotshowcross-reactionwithotherchemicalswithsimilarstructures.Thequantitativeanalysisrangesfrom0.05μg/L-5μg/L(ppb),sensitiveenoughtodetectE1inaspecimenfromriverorwastewater,etc.Witheaseofhandling,thetotaltimeformeasurementisonly2.5hours.TheELISAmeasurementishighlyreproducible;thecoefficientofvariation(CV)isgenerallyunder10%.Thekit,a96-wellmicroplateformat,enablessimultaneousmeasurementofmultiplesamplesatmorereasonablecost.EstroneEstrone(E1)isfoundmoreabundantlyinriverorsewagewaterthan17β-Estradiol(E2),thusrequiringcautiousmonitoring,althoughestrogenicityofE1isreportedtobeweakerthanthatofE2inthevitellogeninsynthesistestinrainbowtrout.E1StandardCurveSamplescontainingEstrone(E1)withinthedynamicrange(0.05μg/L-5μg/L)canbedirectlyappliedtoassayafterfiltration.SampleswithEstronecontentbelowtherangemustbeconcentratedwithsolidphaseextractionpriortotheensuingsession.Coefficientofvariation(CV)isgenerallyunder10%throughoutthedynamicrange.Pretreatment(SimplifiedSolidPhaseExtraction)Alsoseehere1)AdjustthepHofthesampleto5.0with1MAceticacid.2)PoursampleliquidthroughaC18solid-phasecartridge3)WashthecartridgewithdistilledwaterandHexane.4)CoupleasilicacartridgetotheC18cartridgeandeluteE1withamixtureof***ane:acetone=9:1.5)Drythesamplewithnitrogengas,thenadjustMeOHconcentrationat10%formeasurement.Thespecificproceduremayvaryinsamplequality.Cross-reactivityPatternCompoundsCrossreactivity(%)Estrone(E1)1002-methoxyE10.217β-Estradiol(E2)0.316-keto-E20.2E2-17-glucuronide<0.1E2-3-glucuronide<0.1E2-3-sulfate-17-glucuronide<0.1Estriol(E3)<0.116-epi-E3<0.1E3-16-glucuronide<0.1Ethynylestradiol(EE2)<0.1KitFormatKitFormatCommentMicroplate1plate(96wells)&reagentsNeedsamicroplatereader(450nm)Formultipleandsimultaneousmeasurement如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com在线***：***：1069267096MSN：liyuqiu1983@cindylee-1981@联系人：杨先生总机：010-60206266传真：010-60206773www.express-cn.com)