APEELISAKit烷基酚聚氧乙烯醚试剂盒NonionicSurfactantsAPEELISAKitThemonoclonalantibodybindsexclusivelywithAlkylphenolEthoxylate(APE)andAlkylphenolethoxyaceticacid,anddoesnotcross-reactwithothersurfactantsorcompoundsofsimilarstructure.Thedetectionrangeisbetween0.02mg/Land1.0mg/L.Asimpleconcentrationprotocolbasedonsolidphaseextractionisavailabletodeterminemuchlowerconcentration.Witheaseofhandling,thetotaltimeformeasurementisonly2.5hours.Thekit,a96-wellmicroplateformat,enablessimultaneousmeasurementofmultiplesamplesatmorereasonablecost.APEAPEisanabbreviationforAlkylphenolEthoxylate,alsocalledpolyoxyethylenealkylphenylether.APEbelongstononionicsurfactantandisusedmainlyforindustrialdetergentoremulsifier.EthyleneoxidechainsinAPEaresubjecttomicrobialdecomposition,generatingtoxicandhydrophobicbiodegradedcompounds.APEhasrecentlybeenpointedoutasaprecursorofendocrinedisruptingchemicals.Octylphenolethoxylate(N=8)andnonylphenolethoxylate(n=9)aretherepresentativeAPEs.APEStandardCurveSamplescontainingAPEwithinthedynamicrange(0.02mg/L-1.0mg/L)canbedirectlyappliedtoassayafterfiltration.SampleswithAPEcontentbelowtherangemustbeconcentratedwithsolidphaseextractionpriortotheensuingsession.Coefficientofvariation(CV)isgenerallyunder10%throughoutthedynamicrange..Pretreatment(SimplifiedSolidPhaseExtraction)Withthisconcentrationprotocol,APEismeasurablearoundtheorderofpartspertrillion,orng/mL.PoursampleliquidthroughaC18solid-phasecartridgeandeluteAPEwithmethanolforconcentration.ThisprotocolassuresthesimultaneousextractionofAE,anotherPOEtypenonionicsurfactant,andremovalofsubstancesthatmayobstructAPEbinding.Reconstitutetheelutein10%methanolaqueoussolutionafterevaporationfortheELISAassay.Thespecificproceduremayvaryinsamplequality.Cross-ReactivityPatternCompound%reactivityNonionicsurfactantsNPE(Nonylphenolethoxylates)NP20EO(Ave.EOchainlength20)79NP15EO(Ave.EOchainlength15)73NP10EO(Ave.EOchainlength10)100NP5EO(Ave.EOchainlength5)82NP2EO32NP1EO14OPE(Octhylphenolethoxylates)OP20EO(Ave.EOchainlength20)92OP15EO(Ave.EOchainlength15)129OP10EO(Ave.EOchainlength10)200OP5EO(Ave.EOchainlength5)244OP2EO129Nonylphenolethoxyaceticacid(NPnEC)NP2EC267NP1EC200AE(Alkylethoxylates)<0.2AnionicsurfactantsLAS(Linearalkylbenzenesulfonate)<0.2SOAP(Sodiumlaurate)<0.2SDS(Sodiumdodecylsulfate)<0.2AES(Alkylethersulfate)<0.2SubstancesofsimilarstructurePhenol<0.2PEG(Polyethyleneglycol)<0.2ThemonoclonalantibodyhasahighspecificitytoAPEwithvariouspolyethoxylicchainlength(n=1-22)anddoesn'tcross-reactwithothersurfactantsorcompoundsofsimilarstructure.KitFormatKitFormatCommentMicroplate1plate(96wells)&reagentsNeedsamicroplatereader(450nm)Formultipleandsimultaneousmeasurement如有更多其它试剂产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com在线客服：QQ：1069267096MSN：liyuqiu1983@cindylee-1981@联系人：杨先生总机：010-60206266传真：010-60206773www.express-cn.com)