Alkylphenol(AP)ELISAKit***酚APELISAKitThemonoclonalantibodybindsexclusivelywithAlkylphenol(AP),anddoesnotcross-reactwithothersurfactantsorcompoundsofsimilarstructure.Thedetectionrangeisbetween5μg/Land500μg/L.Asimpleconcentrationprotocolbasedonsolidphaseextractionis***ailabletodeterminemuchlowerconcentration.Witheaseofhandling,thetotaltimeformeasurementisonly2.5hours.Thekit,a96-wellmicroplateformat,enablessimultaneousmeasurementofmultiplesamplesatmorereasonablecost.APAPissuspectedasoneoftheendocrinedisruptingchemicalsinmanycountries.Octylphenol(n=8)andnonylphenol(n=9)arewidelyusedasrawmaterialofsurfactantsandanti-oxidants.APisgeneratedbymicrobialdecompositionofethyleneoxidechainsinAPE,whichiswidelyusedasnonionicsurfactant.NPnEC,anotherdecomposedproductofAPE,isalsoassumedashormonemimickingchemicals.APStandardCurveSamplescontainingAPwithinthedynamicrange(5μg/L-500μg/L)canbedirectlyappliedtoassayafterfiltration.SampleswithAPcontentbelowtherangemustbeconcentratedwithsolidphaseextractionpriortotheensuingsession.Coefficientofvariation(CV)isgenerallyunder10%throughoutthedynamicrange.Pretreatment(SimplifiedSolidPhaseExtraction)Ifanextractionandaconcentrationprocessisnotnecessary,addmethanoltothefiltratetobeafinalDMSOconcentrationof1%andmethanolconcentrationof10%(v/v).Ifnot,prepareeachsamplebytheextractionprocedure.[Example]1.RinseaNEXUSSPEsolidphasecartridgeat20ml/min.with***an(10mL)andmethanol(5mL)thenwithdistilledwater(5mL).2.Poursampleliquidthroughthecartridgeat10mL/min.(uptoabout1L)3.Washthecartridgewithdistilledwater(5mL).4.Washthecartridgewith50%methanol(5mL).5.Drythecartridgewithvacuuming.6.Elutetheanalytewithdochloromethane(6mL)thenevaporatethesolventwithnitrogengas.7.Dissolvetheresiduein1%DMSO,10%methanol(1to2mL).8.MeasurethesamplewithAPELISAkit.Cross-reactivityPatternCompound%reactivityNonylphenol(NP)100Octylphenol(OP)96NonylphenolEthoxylate(NPnEO)NPnEO(n=1)1.2NPnEO(n=2)2.1NPnEO(n≒5)3.2NPnEO(n≒7.5)4.5NPnEO(n≒10)4.9OctylphenolEthoxylate(OPnEO)OPnEO(n≒10)2.8NonylphenolEthoxyAceticacid(NPnEC)NPnEC(n=1)0.5NPnEC(n=2)1.5NPnEC(n=3)3.8AnionicSurfactantsLinearAlkylbenzeneSulfonates(LAS)<0.1SodiumDodecylSulfate(SDS)<0.1AlkyletherSulfate(AES)<0.1SodiumLaurate(SORP)<0.1KitFormatKitFormatContentCommentMicroplate96wells&reagentsNeedsamicroplatereader(450nm)Formultipleandsimultaneousmeasurement如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com在线***：***：1069267096MSN：liyuqiu1983@cindylee-1981@联系人：杨先生总机：010-60206266传真：010-60206773www.express-cn.com)