supersensitive AE ELISA Kit醇聚氧乙烯醚***盒
supersensitiveAEELISAKit醇聚氧乙烯醚***盒NonionicSurfactantAEELISAKitThemonoclonalantibodybindsexclusivelywithC10-13AEswithethyleneoxide(EO)chainlength(m=4-25).Thedetectionrangeisbetween3μg/Land150μg/L.Asimpleconcentrationprotocolbasedonsolidphaseextractionis***ailabletodeterminemuchlowerconcentration.Witheaseofhandling,thetotaltimeformeasurementisonly2.5hours.Thekit,a96-wellmicroplateformat,enablessimultaneousmeasurementofmultiplesamplesatmorereasonablecost.AEAEisanabbreviationforAlcoholEthoxylate,alsocalledpolyoxyethylenealkylether.AEbelongstononionicsurfactantandisusedmainlyforresidentialdetergent.ProductionofAEhasbeenincreasingyearbyyearandamountsto140,000t***in1999,whichcorrespondsto30%ofnonionicsurfactants.FoamingabilityofAEisequaltoormorethanthatofanionicsurfactants,whichrequirethecontinuousmonitoringforthecontrolofdrinkingwaterquality.AEStandardCurveSamplescontainingAEwithinthedynamicrange(3μg/L-150μg/Ldissolvedin30%methanol)canbedirectlyappliedtoassayafterfiltration.SampleswithAEcontentbelowtherangemustbeconcentratedwithsolidphaseextractionpriortotheensuingsession.Coefficientofvariation(CV)isgenerallyunder10%throughoutthedynamicrange.Pretreatment(SimplifiedSolidPhaseExtraction)Withthisconcentrationprotocol,AEismeasurablearoundtheorderofpartspertrillion,orng/mL.PoursampleliquidthroughaC18solid-phasecartridgeandeluteAEwithmethanolforconcentration.ThisprotocolassuresthesimultaneousextractionofAPE,anotherPOEtypenonionicsurfactant,andremovalofsubstancesthatmayobstructAEbinding.Rec***titutetheelutein10%methanolaqueoussolutionafterevaporationfortheELISAassay.Thespecificproceduremayvaryinsamplequality.Cross-reactivityPatternreactivityagainsteachAEmolecules(%)Compound%reactivityNonionicSurfactantsAE(C12EO7)100NonylphenolEthoxylate(NP10EO)0.1PolyethyleneGlycol(10EO)<0.1Polyoxyethylenesorbitan(20)monolaurate(Tween-20)2.0AnionicSurfactantsAlkyletherSulfate(AES)13SodiumDodecylSulfate(SDS)1.1SodiumLaurate1.1SopdiumMyristate0.51SopdiumPalmitate0.33SopdiumStearate0.22LinearAlkylbenzeneSulfonates(LASs:C9-C13)<0.1CationicSurfactantsHexadecyltrimethylAmmoniumChloride<0.1KitFormatKitFormatCommentMicroplate1plate(96wells)&reagentsNeedsamicroplatereader(450nm)Formultipleandsimultaneousmeasurement如有更多其它***产品需求与查询,请及时接洽:info@express-cn.com或在我公司查询主页客户留言处(http://www.express-cn.com/expressgb/m)留下您的查询内容,我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com在线***:***:1069267096MSN:liyuqiu1983@cindylee-1981@联系人:杨先生总机:010-60206266传真:010-60206773www.express-cn.com)