LabAssayALPGrade:forCellbiologyStoragecondition:Keepat2-10degreesC.Cat.No.291-58601Size:900testsSummaryAlkalinePhosphataseactivityassaywithp-NitrophenylphosphateasasubstrateAlkalinePhosphatase(ALP)isdistributedinavarietyoftissuessuchasliver,bone,and***allintestineinanimals.Thechangeoftheenzymeactivityintissuesisanim-portanthallmarkforphysiologicalphenomenaasosteo-genesisandsoon.ThiskitisforAlkalinePhosphataseassayinasimultaneousmulti-sampleassayformatwithamicroplateusingp-Nitrophenylphosphateasasubstrate.[KitContents]SubstrateTablet20tablets(p-NitrophenylphosphateDisodium6.7mmol/L,afterdissolving)BufferSolution100mL(2.0mmol/LMgCl2,0.1mol/LCarbonateBuffer,pH9.8)StopSolution100mL(0.2mol/LSodiumHydroxideSolution)StandardSolution10mL(0.5mmol/Lp-NitrophenolSolution)[Assayprinciple]p-Nitrophenylphosphateishydrolyzedintop-NitrophenolandPhosphoricAcidinthecarbonatebuffer(pH9.8)inthepresenceofAlkalinePhosphataseinsample.Releasedp-Nitrophenolshowingyellowcolorisopticallymeasuredat405nmw***elengthastheenzymeactivity.[Features]Dynamicassayrange:>0.06mmol/LStandardassayrange:0~0.5mmol/L(Theactivityatconcentrationmorethan0.5mmol/Lcanbedeterminedbyextrapolationduetoitslinearity.)Reproducibility:C.V.<10%[Reference]Yamamoto,M.,Takahashi,Y.,Tabata,Y.:Biomaterials.24(24),4375(2003).~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com在线***：***：1069267096MSN:liyuqiu1983@cindylee-1981@总机：010-60206266传真：010-60206773www.express-cn.com)