DNA Extractor (R) WB Kit (Sodium Iodide method) (血
DNAExtractor(R)WBKit(SodiumIodidemethod)(血液的***组DNA提取***盒)Grade:forWholeBloodDNAExtractionRegulation/Hazardous:UN:Class3-Flamm.liq.Storagecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.(可应用于除人类以外,马、牛的血液和培养***,细胞等的提取)Cat.No.Pkg.Size291-5050250testsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.TheDNAExtractorWBKitextractsgenomicDNAfromwholeblood,cellculture,andtissue.ItemploysanewextractionprocedureforDNApurificationafterthelysisofcells.Thisprocedure,usingSodiumIodide(NaI)asachaotropicagent,realizesintactDNAisolationofbothhighpurityandhighyield.Furthermore,extractionisdonethroughseveralbriefmicrofugecentrifugati***fromasinglemicrofugetube.DNAextractedwiththiskitissuitableforseveralapplicati***,includingrestrictionenzymedigestion,Southernblotanalysis,andPCR.Thiskitisapplicableto100-1000uLofwholebloodbyproportionallyvaryingthevolumeofsoluti***usedinthestandardmethoddescribedbelow(1mLofwholebloodcanbeappliedbyusinga2mLmicrocentrifugetubeanddoublingtheamountofeachreagent).Wholebloodstoredrefrigeratedfor3weeksdidnotshowasignificantdecreaseintherecoveryofDNAwiththiskit.Ref.:Wang,L.,Hirayasu,K.,Ishizawa,M.,andY.Kobayashi,''PurificationofgenomicDNAfromhumanwholebloodbyisopropanol-fractionationwithconcentratedNaIandSDS'',NucleiAcidsResearch,22(9),1174-75(1994).UN1219andUN1170说明书单询。)