DNAExtractor(R)SPkit（***、血浆的DNA提取***盒）Grade:forGeneticResearchStoragecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.(用于存在于***或血浆中DNA片断的提取)Cat.No.Pkg.Size296-6050150TestsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.DNAextractionfromserumandpla***aDNAExtractorSPKitefficientlyextractsDNAfragmentscontainedinserumandpla***a.BasedontheSodiumIodide(NaI)method1,thiskitenablesthewholeproceduresdoneinasinglemicro-centrifugetubewithoutusinghazardousphenol/chloroform.Recently,tumor-specificgenesh***ebeenamplifiedanddetectedintheserumandpla***aofpatientswithvariousdiseasessuchaslung,breastandcoloncancer.Manyofthesereferencearticlesarecurrentlybeingpublished2,3,4.ThekitisapowerfulpretreatmentreagentforthedetectionandanalysisoftargetDNAbecauseofitshighqualityandyield.FeaturesHighDNAyield(about100%)from***allamountofserumandpla***aTheDNAextractionprocedureisbasedonasimplecentrifugalseparationusingSodiumIodideandalcohol,thatresultsinlesslossofDNA.SafeoperationNophenolorchloroformrequired.Minimumcontamination:TheentireextractionofDNAcanbedoneinasinglecentrifugetubeCompleteremovaloflipidderivedfrombloodLessvariabilityinextractionfromsampletosampleKitContents(50reacti***)Enzymereactionsolution1x10mLProteindigestionsolution1x250uL(uL:micro-L)Sodiumiodidesolution1x15mLAlcoholsolution1x30mLWashingSolution(A)1x50mLWashingSolution(B)1x50mLReferencesIshizawa,M.,Kobayashi,Y.,Miyamura,T.andMatsuura,S.:NucleicAcidsRes.,22,1774(1994).Sozzi,G.,Musso,K.,Ratcliffe,C.,Goldstraw,P.,Pierotti,M.A.andPastorino,U.:Clin.CancerRes.,5,2689(1999)Silva,J.M.,Dominguez,G.,Garcia,J.M.,Gonzalez,R.,VillanuevaM.J.,N***arro,F.,Provencio,M.,San,Martin,S.,Espana,P.andBonilla,F.:CancerRes.,59,3251(1999)Shao,Z.M.,Wu,J.,ShenZ.Z.andNguyen,M.:Clin.CancerRes.,7,2222(2001))