Human/RatBetaAmyloid(42)ELISAKitWako(人/大鼠Beta淀粉质42酶联******盒)Grade:forImmunochemistryStoragecondition:Keepat2-10degreesC.Cat.No.290-62601Size:96TestsSummaryThisisforresearchuseonly.Donotadministerittohuman.ResearchforAlzheimer'sDiseasebetaAmyloidELISAKitsAlzheimer'sDisease(AD)ischaracterizedbythepresenceofextracellularsenileplaques(SPs)andintracellularneurofibrillarytangles(NFT)inthebrain.ThemajorproteincomponentofSPsisbetaAmyloidpeptide(AB)40AND42(43).AB42ismorepronetoaggregatethanAB40.ThereforetheinitialABdepositionbeginswithAB42(43)butnotwithAB40.AB42(43)-positiveandAB40-negativeplaquesmayrepresentearly-stagediffusetypeSPs,andAB40-positiveplaqueappearsintheadvancedstage,especiallymoreofteninthecoredportionofthematureplaque.Inthesekits,weusethemonoclonalantibodieswhichspecificallydetectsAB.ThereforethesekitsaredesignedtobeusedforthequantitativedeterminationofABinsamplessuchastissueculturemedium,tissuehomogenate,CSFandpla***a.FeaturesThesekitsaredesignedtobeusedforthequantitativedeterminationofABinsamplessuchastissueculturemedium,tissuehomogenate,CSFandpla***a.ThesekitsusethemonoclonalantibodiesthatweredevelopedbyTakedaChemicalsIndustries,Ltd.BAN50:SpecificallydetectstheN-terminalofABBNT77:SpecificallydetectstheAB(11-28)ofABBA27:SpecificallydetectstheC-terminalofAB40BC05:SpecificallydetectstheC-terminalofAB42Variationofkit1.HumanbetaAmyloid(1-40)ELISAKitWako(WakoCatalog#292-62301,96tests)ThiskitdetectsthequantityofHumanAB(1-40).Sensitivity*:0.12(pM)Dynamicrange:1.0-100(pM)2.HumanbetaAmyloid(1-40)ELISAKitWako(WakoCatalog#298-62401,96tests)ThiskitdetectsthequantityofHumanAB(1-42).Sensitivity*:0.08(pM)Dynamicrange:1.0-100(pM)3.Human/RatbetaAmyloid(40)ELISAKitWako(WakoCatalog#294-62501,96tests)ThiskitdetectsthequantityofHumanorRat(mouse)AB(x-40)withatruncatedormodifiedN-terminusSensitivity*:0.25(pM)Dynamicrange:1.0-100(pM)4.Human/RatbetaAmyloid(42)ELISAKitWako(WakoCatalog#290-62601,96tests)ThiskitdetectsthequantityofHumanorRat(mouse)AB(x-42)withatruncatedormodifiedN-terminus.Sensitivity*:0.19(pM)Dynamicrange:1.0-100(pM)*:Thesensitivitywasdeterminedbyaddingtwostandarddeviati***tothemeanabsorbanceobtainedwhenthezerostandardwasassayed24times.AssaySummary1.Add100uLofStanda***andSamplesandincubateintherefrigeratorwithplatesealovernight.2.Add100uLoftheHRP-conjugatedAntibodySolution,sealplateandrefrigeratefor1hour.3.Add100uLofTMBSolution,sealplateandincubatefor30minutesatroomtemperatureinthedark.4.Add100uLtheStopSolutionandreadtheabsorbanceofeachwellat450nmwithamicroplatereader.(uL:microliter)ReferencesSuzuki,N.,Cheung,TT.,Cai,XD.,Odaka,A.,Otvos,L.Jr.,Eckman,C.,Golde,TE.andYounkin,SG.:Science,264,1336(1994).Iwatsubo,T.,Odaka,A.,Suzuki,N.,Mizusawa,N.andIhara,Y.:Neuron,13,45(1994).Asami-Odaka,A.,Ishibashi,Y.,Kikuchi,T.,Kitada,C.andSuzuki,N.:Biochemistry,34,10272(1995).FukumotoH.,TomitaT.,MatsunagaH.,IshibashiY.,SaidoT.C.andIwatsuboT.:Neuroreport,10,2965(1999).Scheuner,D.,Eckman,C.,Jensen,M.,Song,X.,Citron,M.,Suzuki,N.,Bird,TD.,Hardy,J.,Hutton,M.,Kukull,W.,Larson,E.,Levy-Lahad,E.,Viitanen,M.,Peskind,E.,Poorkaj,P.,Schellenberg,G.,Tanzi,R.,Wasco,W.,Lannfelt,L.,Selkoe,D.andYounkin,S.:NatureMed.,2,864(1996).Kosaka,T.,Imagawa,M.,Seki,K.,Araki,H.,Sasaki,H.,Tsuji,S.,Asami-Odaka,A.,Fukushima,T.,Imai,K.andIwatsubo,T.:Neurology,48,741(1997).RelatedproductAmyloidbetaProteinImmunohistostainKit(WakoCat#299-56701,50tests)ThiskitstainsAB40orAB42intissuesectionbythemonoclonalantibodiesthatdetectstheportionofC-terminalofAB.AntiPhosphorylatedalphaSynuclein,MonoclonalAntibody(WakoCat#014-20281,50uL)SpecificforhumanalphaSynucleinincludingphosphorylatedSer129.(CloneNo.:pSy#64)AmyloidbetaProtein(1-40),human(WakoCat#019-18761,1mg)AmyloidbetaProtein(1-40)(Hydrochloride),human(WakoCat#014-18951,1mg)AmyloidbetaProtein(1-42),human(WakoCat#016-18771,0.5mg)AmyloidbetaProtein(1-42)(Hydrochloride),human(WakoCat#011-18961,1mg)说明书单询)