Vitellogenin(Carp)ELISAKit(鲤鱼卵黄蛋白原酶联******盒)Storagecondition:Keepat2-10degreesC.Cat.No.300-08751Pkg.Size96testsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.Vitellogenin(Vg)isaprecursorofeggyorkanddetectedfemaleserumnearovulation.Vgisnotdetectedinmaleserum,buttreatedwith17b-estradiol,Vgisdetectableinmaleserum.ManufacturedbyTransGenicInc.(Kumamoto,Japan)CarpVgELISAkitisc***tructedasasandwichELISAformatusinganticarpVgantibodiesandtitrateserumcarpVg.KitContents:(1)Antibody-coatedmicrotiterplate(8wellsx12):1plate(2)Thestandard,CarpVg,lyophilized:2vials(3)Standarddiluent:30mL(4)HRP-conjugatedantiCarpVgantibody(x500):25mL(5)Substratebuffer:15mL(6)OPD(o-Phenylenediamine)tablets:2tablets(7)Washsolution(x20):30mL(8)Stopsolution:6mLRequiredApparatuses:(1)Amicroplatereader(2)Amicropipet(3)AmicroplatewasherPrinciple:Thiskitisc***tructedusingtheantibodiesspecifictocarpVg,asbothcaptureandsecondaryantibodiesofsandwichELISAformat.Duringtheassay,carpVginthesamplesiscapturedbytheantibody,immobilizedonthesurfaceofmicroplatewells,andsimultaneouslyreactswiththehorseradishperoxidase(HRP)labeledantibody.QuantitationofcarpVginsamplescanbemadebymeasurementofHRPactivityofthecomplex,whichisexponentialtothequantityofcarpVg.Features:(1)SensitivityDynamicrange:7.8-500ng/mL(2)RecoveryofexogenousvitellogeninPla***a:***.95.6%ExpiredDate:1monthaftermanufacturing)