S.cerevisiaeDirectTransformationKitWako(酵母S.Cerevisiae直接转化***盒)Grade:forGeneticResearchStoragecondition:Keepat-20degreesC.Cat.No.296-6270120testsCat.No.292-62703100testsCat.No.290-62704500testsSummary.Thisproductisforresearchuseonly.Donotadministerittohuman.forgenome-widescreening!!TheS.cerevisiaeDirectTransformationKitWakoisspeciallydesignedforeasytransformationofthebuddingyeast,Saccharomycessp.TheS.cerevisiaeDirectTransformationProtocolallowssuccessfultransformationsimplybymixingapla***idDNAandthekitreagentswithculturedyeastcells.Nocomplicatedsteps,suchascentrifugationorcellwashing,arerequired.TheS.cerevisiaeDirectTransformationKitWakoisparticularlywellsuitedforhigh-throughputtransformationofalargenumberofyeaststrainsgrownin96-wellplates.Evengenome-widescreening,whichhasbeenproblematic,becomespossible,sincetheS.cerevisiaeDirectTransformationKitWakoeasilytransformsabout4,850strainswithgenedisruption.FeaturesNocentrifugationorwashingstepsarerequired.One-stepprocedurebymixingpla***idDNAandthekitreagentswithculturedyeastcells.Suitablefortransformationofalargenumberofyeaststrainsusing96-wellplates.Applicabletotubeprotocol.Low-viscosityreagentenableshigh-throughputscreening.TransformationEfficiencyBY4743strainwithpRS316pla***id96-wellplateprotocol:500andoverTubeprotocol:5,000andoverTheproductinformationisshownonthepage#10ofWakoProductUpdateNo.15.)