LabAssayALPGrade:forCellbiologyStoragecondition:Keepat2-10degreesC.Cat.No.291-58601Size:900testsSummaryAlkalinePhosphataseactivityassaywithp-NitrophenylphosphateasasubstrateAlkalinePhosphatase(ALP)isdistributedinavarietyoftissuessuchasliver,bone,and***allintestineinanimals.Thechangeoftheenzymeactivityintissuesisanim-portanthallmarkforphysiologicalphenomenaasosteo-genesisandsoon.ThiskitisforAlkalinePhosphataseassayinasimultaneousmulti-sampleassayformatwithamicroplateusingp-Nitrophenylphosphateasasubstrate.[KitContents]SubstrateTablet20tablets(p-NitrophenylphosphateDisodium6.7mmol/L,afterdissolving)BufferSolution100mL(2.0mmol/LMgCl2,0.1mol/LCarbonateBuffer,pH9.8)StopSolution100mL(0.2mol/LSodiumHydroxideSolution)StandardSolution10mL(0.5mmol/Lp-NitrophenolSolution)[Assayprinciple]p-Nitrophenylphosphateishydrolyzedintop-NitrophenolandPhosphoricAcidinthecarbonatebuffer(pH9.8)inthepresenceofAlkalinePhosphataseinsample.Releasedp-Nitrophenolshowingyellowcolorisopticallymeasuredat405nmw***elengthastheenzymeactivity.[Features]Dynamicassayrange:>0.06mmol/LStandardassayrange:0~0.5mmol/L(Theactivityatconcentrationmorethan0.5mmol/Lcanbedeterminedbyextrapolationduetoitslinearity.)Reproducibility:C.V.<10%[Reference]Yamamoto,M.,Takahashi,Y.,Tabata,Y.:Biomaterials.24(24),4375(2003).)