bFGFELISAKitWako(碱性成纤维细胞生长因子bFGF-Elisa***盒)Grade:forbFGFAssayStoragecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.Cat.No.291-51401Size:60TestsSummary[References]Seno,M.,Iwane,M.,Sasada,R.,Moriya,N.,Kurokawa,T.andIgarashi,K.:Hybridoma,8,209(1989)Watanabe,H.,Hori,A.,Seno,M.,Kozai,Y.,Igarashi,K.,Ichimori,Y.andKondo,K.:Biochem.Biophys.Res.Commun.,175,229(1991)Fujimoto,K.,Ichimori,Y.,Kakizoe,T.,Okajima,E.,Sakamoto,H.,Sugimura,T.andTerada,M.:Biochem.Biophys.Res.Commun.,180,386(1991)Nguyen,M.,Watanabe,H.,Budson,A.E.,Richie,J.P.andFolkman,J.:J.Natl.CancerInst.,85,241(1993)Soutter,A.D.,Nguyen,M.,Watanabe,H.andFolkman,J.:CancerRes.,53,5297(1993)Nguyen,M.,Watanabe,H.,Budson,A.E.,Richie,J.P.,Hayes,D.F.andFolkman,J.:J.Natle.CancerInst.,86,356(1994)Thisproductisforresearchuseonly.Donotadministerittohuman.SincediscoveryofbFGF(basicfibroblastgrowthfactor,FGF-2)byGospodarowiczin1974frombovinepituitarygland,humanbFGFhasbeenwellcharacterized:beingabasicproteinwithpI9.6ofMWca.17,000;h***inghighaffinitytobothheparinandheparansulfate;anddistributingadiversityoforgans,tissues,andtumorouscellssuchasrenalcellcarcinoma.Recentinvestigationsurroundingthisproteinhasbeenfocusingonrelati***hipsbetweendiseases,especially,cancersandtheconcentrationofbFGFinpatientserum.Someh***ebeendiscussedaboutco-relationbetweentumorsandbFGFsecretion.However,detectionlimitsonmostofthecommercially***ailablekitsareinadequatecomparedwithbFGFconcentrationinhumanserum.Replyingtocurrentdemands,weh***enewlydevelopedahighlysensitivebFGFELISAKitWakoutilizingmultiplemonoclonalantibodieswell-characterizedwithhumanbFGF.Thekitshouldenabletheuserstomeasuremorethan0.25pghumanbFGFinserumandbiologicalsamplesbythestandardELISAmethod.[Features]Thequantitativerangeof5-500pg/mLhumanbFGFbythestandardassayMinimaldetectionof0.15pgbFGFinserumbFGF-specificquantitationwithoutanyinterferebyserumcomponentsbFGFcanbemeasuredinthecellculturesupernatantNosamplepre-treatmentisrequiredpriortotheassay96dry-wellmicroplateformat[Contents]Antibody-coatedmicroplate(Dry-wellformat)1plateHumanbFGFstandard(Recombinant)1ng/vialHRP-conjugatedFab'(lyophilized)1vialChromogen(o-Phenylenediamine)3tabletsBufferSolutionA30mLBufferSolutionB17mLBufferSolutionC(containing0.02%H2O2)17mLStopSolution(containing1mol/LH2SO4)12mLLog-loggraphpaper1sheetMicroplatesealer2sheets[Principle]Basedonenzyme-linkedimmunosorbantassay(ELISA)withsandwich-typeformat,thekitisdesignedtoquantitatehumanbFGFinserumandbiologicalfluidintherangeof5-500pg/mLusingmultiplemonoclonalantibodieswell-characterizedagainsthumanbFGF.Of96-wellmicroplate,allwellsarecoatedwithamixtureoftwokindofmonoclonalantibodies;cloneNo.52and98withsimilarantigenicrecognitiontostrengthenthecaptureofantigen.Byadditionofspecimenstoantibody-coatedwells,theantigenisspecificallycapturedbythemonoclonalantibodies.Afterwashingoutunboundserumcomponents,HRP-conjugatedFab'preparedfrommonoclonalantibody,cloneNo.3H3,h***inganotherepitoperecognitionisadministeredinthewells,toformsandwich-typecomplexesoftheantibodiesandantigenonthewellsurfaces.ThecapturedbFGFbytheantibodiescanbequantitatedbythechromogenicreactioncatalyzedbyHRPconjugatedwiththeFab'fragment,usingthestandardcurvemadebyhumanbFGFstanda***uppliedwiththekit.[SpecificationSummary]Sensitivity:Max.0.15pg(3pg/mL,samplevolume=50uL)Assayrange:5-500pg/mL(samplevolume=50uL)Linearity:Correlationcoefficientof0.99formeasurementintherangeof5-500pg/mLbyregressionwith3dimensionLog-Log(n=3)Reproducibility:Intra-assay;***erageCV%=<5%(n=5)[Reactivity]ReacttohumanandratbFGF.)