LabAssay(TM)Triglyceride(甘油三酸酯***盒)Grade:forCellbiologyStoragecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.Cat.No.290-63701Pkg.Size1000TestsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.Lipidsinserumc***istoftriglycerides,cholesterols,phospholipids,freefattyacidsandslightamountsoffat-solublecomponentssuchasfatsolublevitaminsandcarotenes.Triglycerides,asmajorcomponentsofverylowdensitylipoprotein(VLDL)andchylomicr***,playanimportantroleinmetaboli***asenergysourcesandtransportersofdietaryfat.LabAssay(TM)TriglycerideisbasedonanenzymaticmethodusingN-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyanilinesodiumsalt(DAOS)asabluepigment.Thiskitisusedforthequantitativedeterminationoftriglyceridesinmouseserum.Itisasimultaneousmulti-sampleassayformatusingamicroplate,butmeasurementscanalsobemadeusingatesttube.[KitContents]Buffer(50mmol/LGood's(PIPES)buffer,pH6.5)3vialsx105mLChromogenSubstrate3vialsxfor105mL(whenrec***tituted)Lipoproteinlipase99units/mLAdenosine5'-TriphosphateDisodiumSaltTrihydrate(ATP)1.7mmol/LGlycerolkinase30units/mLGlycerol-3-phosphateOxidase(GPO)1.0unit/mLPeroxidase(POD)5.5units/mLN-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyanilinesodiumsalt(DAOS)0.54mmol/L4-Aminoantipyrine0.11mmol/LAscorbateOxidase4.5units/mL(beforerec***titute)ContainsSodiumHydroxide8.01%.StandardSolution1vialx10mLGlycerol31.2mg/dL(Correspondingto300mg/dLTriolein)[Assayprinciple]Triglyceridesinasamplearehydrolyzedtoglycerolandfreefattyacidsinareactioncatalyzedbylipoproteinlipase(LPL).Glycerolisconvertedtoglycerol-3-phosphatebyglycerolkinase(GK)inthepresenceofATP.Glycerol-3-phosphatesoformedisoxidizedbyglycerol-3-phosphateoxidase(GPO)inareactionthatproduceshydrogenperoxide.ThehydrogenperoxideproducedcausesDAOSand4-Aminoantipyrinetoundergoaquantitativeoxidativecondensationcatalysedbyperoxidase(POD),producingabluepigment.Theamountoftriglyceridescontainedinthesampleisdeterminedbymeasuringtheabsorbanceofthebluecolor.[Performance]Sensitivity(assayinatesttube)Theabsorbanceisbelow0.10,whenmeasuringpurifiedwaterasasample.Theabsorbanceis0.09to0.25,whenmeasuring300mg/dLtriglyceridesasasample.SpecificityThetriglyceridesconcentrationislessthan&plu***n;12%,whenmeasuringtheknownconcentrationofcontrolserumasasample.[Expirationdate]12monthsafterthemanufacture[Reference]Spayd,R.W.,Bruschi,B.etal.:Clin.Chem.,24,1343(1978).)