RatGlucagonELISAKitwako（大鼠胰***素***盒）Grade:forDiabetesResearchStoragecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.Cat.No.Pkg.Size297-5710196TestsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.[Principleoftheassay]MixtureofbiotinylatedratPancreaticGlucagonandthatinthesampleorthestandardmaterialbindstorabbitantibodyspecifictoratPancreaticGlucagoncoatedonthemicroplatewellsurfaceincompetitivequentialreactionwithHRP-conjugatedstrept***idinresultsinaformationofHRP-strept***idin-biotin-antibodycomplexinthewell,whichcatalyzeshydrogenperoxide,generatingcolorbyoxidationofanacceptorsubstrate.[KitContents]Antibody-coatedMicrotiterPlate1plate(AntiRatGlucagon,Rabbit)RatGlucagonStandard10ngBiotinylatedRatGlucagonfor6mLHRP-conjugatedStrept***idin12mLChromogen(OPDTablet)2tabletsChromogenDiluentSolution26mLBufferA10mLBufferB15mLWashStockSolution(20x)50mLStopSolution(1mol/LH2SO4)12mLAdhesivePlateCover4covers[Capability]Dynamicrange:50~10,000pg/mLCross-reactivity:Specifictorat,mouse,andhumanpancreaticGlucagon,butnotcross-reactivetointestinalGlucagonnorGlucagon-likepeptidesasGLP-1andGLP-2.Recoverydata:101~108%说明书单询。)