RatLeptinELISAKit(大鼠瘦素Elisa***盒)Grade:forAdiposityResearchStoragecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.Cat.No.Pkg.Size297-5760196TestsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.[Principleoftheassay]Thiskitisasandwich-formatofELISAwithtwoantibodiesspecifictoratleptin.Monoclonalantibodytoleptiniscoatedonthemicroplatewell,whichcapturesleptininsample.FollowingreactionwithHRP-conjugatedrabbitantiratleptinantibody,formsHRPlabeledantigen-antibodycomplexonthewell,whichreportsthesignalofleptinamountinthesamplebychromogenicreactionassociatedwithcatalysisofhydrogenperoxide.[KitContents]Antibody-coatedMicrotiterPlate1plate(AntiRatLeptin,monoclonalantibody)RatLeptinStandard20ngHRP-conjugatedAntibody,rabbit6mLChromogen(OPDTablet)2tabletsChromogenDiluentSolution24mLWashStockSolution(20X)50mLBufferA20mLBufferB20mLStopSolution(1mol/LH2SO4)12mLAdhesivePlateCover2covers[Capability]DynamicrangeA.Samplesforserumandpla***a:312.5~20,000pg/mLB.Samplesforothersthanserumandpla***a:78.1~5,000pg/mLCross-reactivity:SpecifictoratLeptin,butnothardlycross-reactivetohumanLeptinRecoverydata:93~100%(serum);92~106%(culturesupernatants))