RatC-PeptideELISAKit（大鼠C肽Elisa***盒）Grade:forDiabetesResearchStoragecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.Cat.No.Pkg.Size295-5740196TestsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.[KitContents]AntibodycoatedMicrotiterPlate:1plateRatC-PeptideStandard:50ngBiotinylatedRatC-Peptide:for8mLAntiRatC-Peptide,Rabbit:12mLHRP-conjugatedStreptzvidin:12mLChromogen(OPDTablet):2tabletsWashStockSolution(20x):50mLBuffer:35mLStopSolution(1mol/LH2SO4):12mLAdhesivePlateCover:3covers[Principe]Onthesurfaceofthemicroplatewells,goatantirabbitIgGiscoatedandwhensampleisreactedwithrabbitandInsC-PeptideandbiotinylatedratInsC-peptideinthewell,thegoatantibodycapturesthecomplexoftherabbitantibodyandratInsC-peptideformedincompetitivereaction.Subsequentreactionwithhorseradishperoxides(HRP)-conjugatedStreptzvidintothecomplexonthewellsurfaceresultsinlabelingofthecomplexwithHRPwhichgeneratesthesignalforthepresenceofratInsC-peptideinthesample.[Capability]Dynamicrange:0.56-50ng/mLCross-reactivity:SpecifictoratInsC-Peptide,butnotreactivetoeitherthecoorespondingoftheotheranimal.Recoverydata(serum):101-107%说明书单询。)