BGSTARBKit(β-葡聚糖***盒B)Storagecondition:Keepat2-10degreesC.Manufacturer:MaruhaNichiroFoods,inc.Cat.No.636-01061Size:20testsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.Amebocytelysateofhorseshoecrabsishighlyreactivetoendotoxinandhasbeenusedasanendotoxindetectingreagentinvariousfields.Thelysateisalsoreactivetobeta-glucan.Apurifiedfractionispreparedwhentheendotoxinreactivefactorsandcoagulogenareremovedfromthelysate.Themainreagentinthiskitisthepurifiedfractionaddedwiththechromogenicsyntheticsubstrate.Thisreagentisnotreactivetoendotoxinandspecificallydetectsbeta-glucan.PrincipleofthetestThefactorGinthepurifiedfractionofthelysatereactswith(1->3)-beta-D-glucan.ThenthefactorGcascadereacti***areactivated.Theclottingenzymeproducedinthecascadereacti***reactswiththesubstratetoreleasep-nitroaniline.Thereleasedp-nitroanilineiscoupledwiththediazocouplingreagent.Thentheabsorbanceofthereactionmixtureismeasured.Theamountofthep-nitroanilineisquantitativelydetermined.BGSTARcanbeusedonamicroplatereaderbytheendpointmethodorthekineticmethod.Intheendpointmethod,highersensitivitycanbeobtainedasthereleasedp-nitroanilineiscoupledwiththediazoreagentandtheabsorptivityisenhanced.Inthekineticmethod,anoperatorcanle***eafterstartingthereactionandthemeasurablerangeiswider.Orderinginformation639-01051BGSTARAKITContents:(1)MainReagent,1vialx2.2mL,(2)BufferSolution,1vialx3.0mL,(3)StandardCurdlan,1vialxapprox.1,000pg,(4)beta-Glucan-FreeWater,1vialx15mL,(5)DiazocouplingReagent,1vialx4.8mg,(6)SolventforDiazocouplingReagent1,1vialx12mL,(7)DiazocouplingReagent2,1vialx36mg,(8)DiazocouplingReagent3,1vialx8.4mg636-01061BGSTARBKITContents:(1)MainReagent,1vialx2.2mL,(2)BufferSolution,1x3.0mLNote:WithonevialofMainReagent,40testscanbeperformedonamicroplatereader.Forthekineticmethod,DiazocouplingReagentsarenotused.)