Amyloidbeta-ProteinImmunohistostainKit(β淀粉样蛋白******染色***盒)Storagecondition:Keepat2-10degreesC.Manufacturer:WakoPureChemicalIndustries,Ltd.Cat.No.Pkg.Size299-5670150TestsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.Theplaqueformationofamyloidb-protein(Ab)inthebrainisoneofwell-knownsymptomsofAlzheimer'sdiseasewhicharereportedtocauseneuraldeath.ThiskitallowsdistinctivestainbetweentheplaquesofAb40(1-40)andAb42(1-42)inasectiontoeachother.Features:(1)Distinctivehisto-stainingofAbplaquesofAb40andAb42inAlzheimer'sdiseasedbraintissues.(2)Ahighsensitivityhisto-immunedetectionofAbplaquesintissuesecti***withlowbackgroundKitcontents:(1)BlockingSerum:10mL(2)AntiMouseIgG(H+L),Goat,BiotinConjugated:10mL(3)ABCSolution(Strept***idin-biotin-peroxidasecomplex):10mL(4)FormicAcid(90%):15mL(5)AntiAmyloidb-Protein(1-40),Monoclonal(CloneNo.:BA27):7mL(6)AntiAmyloidb-Protein(1-42),Monoclonal(CloneNo.:BC05):7mL(7)Trypsin,Crystalline:50mgPrinciples:TheantigenintissuesectionissequentiallyreactedwiththeprimaryantibodyspecifictoeitherAb40orAb42,andbiotinylatedantimouseIgGasthesecondaryantibodyandABCreagentinthisorder,resultinginformationofanantige-antibodies-ABCcomplex.ByadditionofhydrogenperoxideandDAB,asubstrateandanacceptorofperoxide,theperoxidaselinkingtothecomplexcatalysishydrogenperoxidetoformoxidizedDABbrownprecipitateinthelimitedareaoftheplaqueslocalizinginthetissuewhichcanbeobservedbyanopticalmicroscope.References:(1)Iwatsubo,T.,Odaka,A.,Suzuki,N.,Mizusawa,H.,Nukina,N.andIhara,Y.:Neuron,13,45(1994).(2)Saido,TC.,Iwatsubo,T.,Mann,DMA.,Ihara,Y.,Shimada,H.andKawashima,S.:Neuron,14,457(1995).(3)Odaka,A.,Ishibashi,Y.,Kikuchi,T.,Kitada,C.andSuzuki,N.,:Biochemistry,34,10272(1995).(4)Fukumoto,H.,Odaka,A.,Suzuki,N.,Shimada,H.,Ihara,Y.andIwatsubo,T.:Am.J.Pathol.,148,259(1996).(5)Fukumoto,H.,Odaka,A.,Suzuki,N.andIwatsubo,T.:Neurodegeneration,5,13(1996).)