microRNACloningKitwakoGrade:forGeneticResearchRegulation/Hazardous:UN:Class8-CorrosiveStoragecondition:Keepat-20degreesC.Cat.No.290-66501Size:8TestsSummaryThisproductisforresearchuseonly.Donotadministerittohuman.ThemicroRNACloningKitWakocanpreparecDNAencodingmicroRNAiscompletedwithin1.5daysafterextractionof***allRNAfractionbyusingdenaturedpolyacrylamidegelandspincolumnextraction.TheexistingmethodsofmicroRNAcloningh***efollowingsomeproblems,severalextractionbydenaturedpolyacrylamidegel,manystepsofRNAhandling,useofRI,useofexpensiveapparatussuchasanimaginganalyzer.ThemicroRNACloningKitWakoissupportedbyshrimpalkalinephosphatase(SAP),thermostablesinglestrandDNAligase(sellingseparately),andoriginalmodifiedadaptors.Thecloningefficiencyusingthiskitisimprovedhigherthanthatoftheconventionalmethods,whichusedbacterialalkalinephosphataseandT4RNAligase.Note:ThiskitmustbeusedtogetherwithSingleStrandDNALigase,Thermostable(WakoCat.#298-65103,200units),whichissoldseparatelyasastand-aloneproduct.ThisligasewillbeusedforligationofssDNA-ssDNA,ssRNA-ssRNAandssDNA-ssRNAdependingonATP.Reactionofthisligasein55-65degrees,whichistheoptimumreactiontemperature,willpromotetheligationefficiency.FeaturesHighcloningefficiencyCloningofsecondarystructuredmicroRNAHighreproducibilityofmicroRNAcloningKitContents(for8reacti***)SAP:1vialx16uL5xSAPBuffer:1vialx64uL40xLigationBuffer:1vialx16uLRNaseInhibitor:1vialx16uL10mmol/LMnCl2:1vialx16uLReverseTranscriptase:1vialx8uL10xRTBuffer:1vialx16uLdNTPMixture:1vialx112uL0.5mol/LEDTA,pH8.0:1vialx16uL1mol/LTris-HCl,pH7.5:1vialx160uLEthachinmate:1vialx24uL10mol/LAmmoniumAcetate:1vialx960uL3'Adaptor(50pmol/uL):1vialx8uL5'Adaptor(50pmol/uL):1vialx8uLRTPrimer(50pmol/uL):1vialx8uL5'PCRPrimer(50pmol/uL):1vialx16uL3'PCRPrimer(50pmol/uL):1vialx16uLControlRNA(30ng/uL):1vialx8uLmicroRNACloningKitWakoispatentpending.(1,10,2007)[RelatedProducts]WakoCatalogNo.DescriptionPackageSize015-22411AntiAgo1,MonoclonalAntibody(2A7)50uL014-22023AntiMouseAgo2,MonoclonalAntibody50uL018-22021ditto100uL011-22033AntiHumanAgo2,MonoclonalAntibody50uL015-22031ditto100uL292-66701microRNAIsolationKit,HumanAgo210reacti***292-67301microRNAIsolationKit,MouseAgo210reacti***290-66501microRNACloningKitWako8reacti***298-65103SingleStrandDNALigase,thermostable,recombinant,Solution200units292-65101ditto500unitsuL:microlitters说明书单询)