小鼠甘露聚糖结合凝集素相关丝氨酸蛋白酶(MASP)ELISA Kit(mannan binding
SPECIMENCOLLECTION\PROCESSINGANDSTORAGElSerum---***oidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serumshouldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.lPla***a---EDTA\citrateandheparinpla***acanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestpla***a.lCellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.lStorage---Ifnotanalyzedshortlyaftercollection,samplesshouldbealiquoted(250-500ul)to***oidfreeze-thawcyclesandstoredfrozenat-70℃.***oidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,***oiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,thisshouldberemovedpriortoassaybycentrifugationorfiltration.Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbefo说明书Email:info@索取;13801829628定货；HR-M0414)