MonoclonalAntibodytoMicrocystin-LR(MC10E7)ProductSpecificationCLONE:MC10E7ISOTYPE:MouseIgG1PURITYDETAIL:ProteinG-affinitypurified.FORMULATION:Lyophilized.REC***TITUTION:Rec***titutewith200μlPBScontainingsodiumazide.IMMUNOGEN:Microcystin-LRlinkedviaN-methyl-dehydroalaninetocationizedovalbumin.SPECIFICITY:Recognizesall4-Argmicrocystins.APPLICATION:ELISA(1:10'000-1:100'000)Microcystinconcentrati***canbemeasuredintherangeof~0.006μg/lto0.3μg/l.TheELISAsensitivityisabout1'000timeshighercomparedtoUVdetection.SHIPPING:SHIPPEDONBLUEICELONGTERMSTORAGE:-20°CUSE/STABILITY:Storageat4°Cwilldecreaseantibodyactivityandmustbe***oided.HANDLING:***oidfreeze/thawcycles.Afteropening,preparealiquotsandstoreat-20°C.ProductDescriptionWithadirectcompetitiveenzyme-linkedimmunosorbentassay(ELISA)usingantibodyMC10E7,IC50valuesformicrocystin-LRof0.06μg/lh***ebeenobtained.TheprovisionalguidelinevalueproposedbytheWHOis1μg/lfordrinkingwater.Thedetectionlimitformicrocystin-LRis0.006μg/l.Allmicrocystinvariantscontaininganarginineatposition4showsimilarIC50valuesanddetectionlimits,whereasothermicrocystins,suchasmicrocystin-LAarenotrecognized.Theaffinityc***tantforMC10E7wasdeterminedtobeatleast7x1010l/mol.Theantibodywastestedforitsrobustnessagainstinterferences(humicacids,pH,saltcontent,surfactantsororganicsolvents)andwasfoundtobeverystable.Microcystin-LRspikedwatersamplesintheconcentrationrangebetween0.01and0.1μg/lweremeasuredandameanrecoveryof99.9&plu***n;16.4%wasfound.Theantibodyiswellsuitedforthesensitiveanalysisofmicrocystinsindrinkingaswellassurfacewater.ProductSpecificLiteratureReferencesHighlysensitiveimmunoassaybasedonamonoclonalantibodyspecificfor[4-arginine]microcystins:A.Zeck,etal.;Anal.Chim.Acta441,1(2001)Developmentofadirectcompetitivemicrocystinimmunoassayofbroadspecificity:M.G.Weller,etal.;Anal.Sci.17,1445(2001)AbstractGeneralInformationMicrocystinsareextremelytoxiccompoundsproducedbycyanobacteria(blue-greenalgae),belongingtospeciesofMicrocystis,Oscillatoria,AnabaenaandNostoc.Thecontaminationofdrinkingwaterorwaterofrecreationalareascancauseseverehealthproblemstoexposedhumansandanimals.Microcystinspossessacyclicheptapeptidestructureofthegeneralcompositioncyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla),whereAddaistheunusualC20aa3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoicacidandXandYarevariableL-aa.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com总机：010-60206266传真：010-60206773www.express-cn.com)