酪胺酸酶Tyrosinase from mushroom
酪胺酸酶TyrosinasefrommushroomBioChemika,lyophilized,≥2000units/mgDescripti***OtherNotesKineticstudyofthe***inactivationoftyrosinase1;ChromogenicassayUnitDefinition1Ucorrespondstotheamountofenzymewhichincreasestheabsorbanceat305nmby0.001perminuteatpH6.5and25°C(L-tyrosineassubstrate,3.0mLreactionmix);30absorbance-Uasdescribedaboveareequivalentto~1U[when1Uistheamountofenzymewhichoxidyzes1μmol4-methylcatecholperminuteatpH6.5and25°C]GeneraldescriptionIsoelectricpoint(pI):4.7-5pHoptimumis6-7Molecularweight:128kDabysedimentationvelocitydiffusion;133kDabylight-scatteringmeasurements,and119.5kDabyelectrophoresis.Tyrosinaseisacopper-containingoxidase,whichhasactivityforbothcatecholsandcresol.Itisresp***ibleforbrowningreacti***.Theenzymeisreportedtoh***etwobindingsitesforaromaticsubstratesandadifferentbindingsiteforoxygen-copper.ThecopperisprobablyCu(I),withinactivationinvolvingoxidationtoCu(II)ion.PropertiesmolwtMr~125000solubility0.02Mphosphate,pH6.5:5mg/mL,clear,slightlybrowntodeepbrownstoragetemp.?20°C~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~如有更多其它***产品需求与查询,请及时接洽:info@express-cn.com或在我公司查询主页客户留言处(http://www.express-cn.com/expressgb/m)留下您的查询内容,我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com总机:010-60206266传真:010-60206773www.express-cn.com)
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