β－葡聚糖***盒BGSTARAKitandBGSTARBKitManufacturer:WakoPureChemicalIndustries,Ltd.BGSTARAKitProductCode:639-01051Package:20testsStorage:keepat2-10degreeCSummaryThisproductisforresearchuseonly.Donotadministerittohuman.Amebocytelysateofhorseshoecrabsishighlyreactivetoendotoxinandhasbeenusedasanendotoxindetectingreagentinvariousfields.Thelysateisalsoreactivetobeta-glucan.Apurifiedfractionispreparedwhentheendotoxinreactivefactorsandcoagulogenareremovedfromthelysate.Themainreagentinthiskitisthepurifiedfractionaddedwiththechromogenicsyntheticsubstrate.Thisreagentisnotreactivetoendotoxinandspecificallydetectsbeta-glucan.PrincipleofthetestThefactorGinthepurifiedfractionofthelysatereactswith(1->3)-beta-D-glucan.ThenthefactorGcascadereacti***areactivated.Theclottingenzymeproducedinthecascadereacti***reactswiththesubstratetoreleasep-nitroaniline.Thereleasedp-nitroanilineiscoupledwiththediazocouplingreagent.Thentheabsorbanceofthereactionmixtureismeasured.Theamountofthep-nitroanilineisquantitativelydetermined.BGSTARcanbeusedonamicroplatereaderbytheendpointmethodorthekineticmethod.Intheendpointmethod,highersensitivitycanbeobtainedasthereleasedp-nitroanilineiscoupledwiththediazoreagentandtheabsorptivityisenhanced.Inthekineticmethod,anoperatorcanle***eafterstartingthereactionandthemeasurablerangeiswider.Orderinginformation639-01051BGSTARAKITContents:(1)MainReagent,1vialx2.2mL,(2)BufferSolution,1vialx3.0mL,(3)StandardCurdlan,1vialxapprox.1,000pg,(4)beta-Glucan-FreeWater,1vialx15mL,(5)DiazocouplingReagent,1vialx4.8mg,(6)SolventforDiazocouplingReagent1,1vialx12mL,(7)DiazocouplingReagent2,1vialx36mg,(8)DiazocouplingReagent3,1vialx8.4mg636-01061BGSTARBKITContents:(1)MainReagent,1vialx2.2mL,(2)BufferSolution,1x3.0mLNote:WithonevialofMainReagent,40testscanbeperformedonamicroplatereader.Forthekineticmethod,DiazocouplingReagentsarenotused.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com总机：010-60206266传真：010-60206773www.express-cn.com)