Vitellogenin(Carp)ELISAKitWakoStoragecondition:Keepat2-10degreesC.Manufacturer:TransGenicInc.SummaryThisproductisforresearchuseonly.Donotadministerittohuman.Vitellogenin(Vg)isaprecursorofeggyorkanddetectedfemaleserumnearovulation.Vgisnotdetectedinmaleserum,buttreatedwith17?-estradiol,Vgisdetectableinmaleserum.ManufacturedbyTransGenicInc.(Kumamoto,Japan)CarpVgELISAkitisc***tructedasasandwichELISAformatusinganticarpVgantibodiesandtitrateserumcarpVg.KitContents:(1)Antibody-coatedmicrotiterplate(8wellsx12):1plate(2)Thestandard,CarpVg,lyophilized:2vials(3)Standarddiluent:30mL(4)HRP-conjugatedantiCarpVgantibody(x500):25?L(5)Substratebuffer:15mL(6)OPD(o-Phenylenediamine)tablets:2tablets(7)Washsolution(x20):30mL(8)Stopsolution:6mLRequiredApparatuses:(1)Amicroplatereader(2)Amicropipet(3)AmicroplatewasherPrinciple:Thiskitisc***tructedusingtheantibodiesspecifictocarpVg,asbothcaptureandsecondaryantibodiesofsandwichELISAformat.Duringtheassay,carpVginthesamplesiscapturedbytheantibody,immobilizedonthesurfaceofmicroplatewells,andsimultaneouslyreactswiththehorseradishperoxidase(HRP)labeledantibody.QuantitationofcarpVginsamplescanbemadebymeasurementofHRPactivityofthecomplex,whichisexponentialtothequantityofcarpVg.Features:(1)SensitivityDynamicrange:7.8-500ng/mL(2)RecoveryofexogenousvitellogeninPla***a:***.95.6%ExpiredDate:1monthaftermanufacturing~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~如有更多其它***产品需求与查询,请及时接洽：info@express-cn.com或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/m）留下您的查询内容，我们即会在当天为您作出回复。伊普瑞斯科技有限公司info@express-cn.com总机：010-60206266传真：010-60206773www.express-cn.com)