AMVTranscriptase(反转录酶)编号规格售价￥ZP00701100U250ZP00702500U1000ZP007031000U1800相关***抽提***盒...反转录***盒...dNTPs...Taq酶...DEPC...枪头、离心管...1、AMVReverseTranscriptase5XReactionBuffer:TheAMVReverseTranscriptase5XReactionBuffersuppliedwiththisenzymehasacompositionof250mMTris-HCl(pH8.325°C),250mMKCl,50mMMgCl2,2.5mMspermidineand50mMDTT.2、EnzymeStorageBuffer:AMVReverseTranscriptase(AMV-RT)issuppliedin200mMpotassiumphosphate(pH7.24°C),0.2%Triton?X-100,2mMDTTand50%glycerol.3、Source:Purifiedfrom***ianmyeloblastosisvirusparticles.4、StorageConditi***:Storeat–20°C.***oidmultiplefreeze-thawcyclesandexposuretofrequenttemperaturechanges.5、UnitDefinition:Oneunitisdefinedastheamountofenzymerequiredtocatalyzethetransferof1nmolofdeoxynucleotideintoacid-precipitablematerialin10minutesat37°C.Thereactionconditi***are:50mMTris-HCl(pH8.3),40mMKCl,8.75mMMgCl2,10mMDTT,0.1mg/mlacetylatedBSA,1mMradiolabeleddTTPand0.25mMpoly(A):oligo(dT).6、UsageNotes:TheAMVReverseTranscriptase5XReactionBufferisintendedforuseinstandardfirst-strandcDNAsynthesisreacti***.Nodeoxynucleotidesareinthebuffer;TheformulationofAMVReverseTranscriptase5XReactionBufferisnotcompatiblewithM-MLVReverseTranscriptase.Upto10μlofanRTreactioncontainingAMV-RTandthesuppliedAMVReverseTranscriptaseReactionBuffercanbeaddedtoPCRamplificationreacti***thatuseTaqDNAPolymerase.详情请登入http://.cn或来电021-54460832GensyntheseTaqDNAPolymeraseTaqPolymeraseGeneSynthesisDNASynthesePeptideSynthesis全***合成***合成密码子优化细胞培养细胞转染细胞稳转细胞瞬转)