FicoScript®M-MLVTranscriptase(反转录酶)编号规格售价￥ZP006011000U150ZP006025000U500ZP0060310000U800相关***抽提***盒...反转录***盒...dNTPs...Taq酶...DEPC...枪头、离心管...FicoScript®ReverseTranscriptase（反转录酶）isanengineeredversionofM-MLVRTwithreducedRNaseHactivityandincreasedthermalstability.TheenzymeispurifiedtonearhomogeneityfromE.colicontainingthemodifiedpolgeneofMoloneyMurineLeukemiaVirus.Theenzymecanbeusedtosynthesizefirst-strandcDNAattemperaturesupto48°C,providingincreasedspecificity,higheryieldsofcDNA,andmorefulllengthproductthanotherreversetranscriptases.ItcangeneratecDNAfrom100bpto>12kb.ItissuitableforqPCR.1、EnzymeStorageBuffer:M-MLVReverseTranscriptaseissuppliedin20mMTris-HCl(pH7.5),200mMN***,0.1mMEDTA,1mMDTT,0.01%NonidetP-40and50%glycerol.2、M-MLVReverseTranscriptase5XReactionBuffer:WhentheM-MLVReverseTranscriptase5XReactionBuffersuppliedwiththisenzymeisdiluted1:5,ithasacompositionof50mMTris-HCl(pH8.325°C),75mMKCl,3mMMgCl2and10mMDTT.3、Source:PurifiedfromanE.colistrainexpressingarecombinantclone.4、StorageConditi***:Storeat–20°C.***oidexposuretofrequenttemperaturechanges.5、UnitDefinition:Oneunitisdefinedastheamountofenzymerequiredtocatalyzethetransferof1nmolofdeoxynucleotideintoacid-precipitablematerialin10minutesat37°C.Thereactionconditi***are:50mMTris-HCl(pH8.3),7mMMgCl2,40mMKCl,10mMDTT,0.1mg/mlBSA,0.5mM[3H]dTTP,0.025mMoligo(dT)50,0.25mMpoly(A)400and0.01%NP-40.6、UsageNote:M-MLVReverseTranscriptaseislessprocessivethanAMVReverseTranscriptase,andtherefore,moreunitsoftheM-MLVenzymearerequiredtogeneratethesameamountofcDNAasintheAMVreaction.Thus,startingwith1μgofmRNAinafirst-strandcDNAsynthesis,200unitsoftheM-MLVenzymearerecommendedasopposedto25unitsoftheAMVenzyme.反转录酶说明书***（大宗用户价格面议，电话：021－54460831）GensyntheseTaqDNAPolymeraseTaqPolymeraseGeneSynthesisDNASynthesePeptideSynthesis全***合成***合成密码子优化细胞培养细胞转染细胞稳转细胞瞬转)