epicentreRT71225MasterAmp™RT-PCRKitforHighSensitivity产品简介：北京中北林格供应epicentreRT71225MasterAmp™RT-PCRKitforHighSensitivity，中北林格是epicentre总代理，提供epicentre产品货号报价查询。其他包括lucigen总代理，cellscript总代理，Biosearchtechnologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>产品名称:MasterAmp™RT-PCRKitforHighSensitivity货号:RT71225规格:25Rxns存储温度:-20℃品牌：lucigen/epicentre</p>供应商：中北林格</p>产地：us</p>发货地：北京</p>epicentreRT71225MasterAmp™RT-PCRKitforHighSensitivity产品描述应用领域High-sensititivitydetectionofRNA,includingRNApresentatlowcopynumber.高灵敏度检测RNA，包括低拷贝数的RNA。RT-PCRapplicationswheresensitivityistheprimaryconcern.RT-PCR应用中，灵敏度是最主要的问题。MultiplexRT-PCR.多重RT-PCR。用于高灵敏度的MasterAmp™RT-PCR试剂盒是一种单酶，单管RT-PCR系统（图1）。它能够从低至1pg的细胞总RNA中扩增RNA（图2）。它是扩增低拷贝数RNA转录本的理想选择。TheMasterAmp™RT-PCRKitforHighSensitivityisasingleenzyme,single-tubeRT-PCRsystem(Fig.1).ItiscapableofamplifyingRNAfromaslittleas1pgoftotalcellularRNA(Fig.2).Itisidealforamplifyinglow-copy-numberRNAtranscripts.用于高灵敏度的MasterAmpRT-PCR试剂盒使用RetroAmp™RTDNA聚合酶，这是一种热稳定酶，在≥60°C时具有峰值逆转录酶活性，而没有RNaseH活性。较高温度下的逆转录减少RNA二级结构，并增加cDNA合成的特异性和敏感性。由于RT和PCR均发生在单个试管中，因此该试剂盒的灵敏度进一步提高。RT期间合成的所有cDNA均用作PCR扩增的模板。TheMasterAmpRT-PCRKitforHighSensitivityusesRetroAmp™RTDNAPolymerase,athermostableenzymethathaspeakreversetranscriptaseactivityat≥60°CandlacksRNaseHactivity.ReversetranscriptionathighertemperaturesreducesRNAsecondarystructureandincreasesthespecificityandsensitivityofcDNAsynthesis.ThesensitivityofthiskitisfurtheraugmentedbecausebothRTandPCRoccurinasingletube.AllcDNAsynthesizedduringRTisusedasatemplateforPCRamplification.该试剂盒还整合了具有甜菜碱的MasterAmpPCR增强剂*，可通过减少DNA合成过程中的停顿和终止来进一步提高RT-PCR的特异性，一致性和敏感性。ThekitalsoincorporatesMasterAmpPCREnhancerwithbetaine,*whichfurtherimprovesthespecificity,consistency,andsensitivityofRT-PCRbyreducingpausesandstopsduringDNAsynthesis.注意：尽管用于高灵敏度的MasterAmpRT-PCR试剂盒提供了无与伦比的RT-PCR灵敏度，但当高保真度最高时，应使用MasterAmp高保真RT-PCR试剂盒通过RT-PCR扩增RNA进行克隆，测序或表达。重要。Note:AlthoughtheMasterAmpRT-PCRKitforHighSensitivityprovidesunsurpassedRT-PCRsensitivity,theMasterAmpHighFidelityRT-PCRKitshouldbeusedtoamplifyRNAbyRT-PCRforcloning,sequencing,orexpression,whenhighfidelityismostimportant.优势说明少至1pg的细胞总RNA即可进行高灵敏度RT-PCR（图2）。优于其他供应商的同类产品。High-sensitivityRT-PCRfromaslittleas1pgoftotalcellularRNA(Fig.2).Outperformssimilarproductsfromothervendors.ThermostableRetroAmp™RTDNAPolymeraseenablesRTtobeperformedathighertemperature,whichminimizesRNAsecondarystructure.Useofoneenzymeinaone-stepandone-tubereactionwithnosampletransfersreducesthechanceforcross-contamination.TheMasterAmpPCREnhancertechnologyincreasesRT-PCRsensitivityandspecificity.ThermostableRetroAmp™RTDNA聚合酶可使RT在更高的温度下进行，从而最大程度地减少了RNA二级结构。一种酶的一步和一管式反应，无需样品转移，可减少交叉污染的机会。MasterAmpPCREnhancer技术可提高RT-PCR的灵敏度和特异性。epicentreRT71225MasterAmp™RT-PCRKitforHighSensitivity图例说明图1.可以与MasterAmp™高保真RT-PCR试剂盒一起使用的不同RT-PCR方案。Figure1.DifferentRT-PCRprotocolsthatcanbeusedwiththeMasterAmp™HighFidelityRT-PCRKit.图2.使用高灵敏度的MasterAmp™RT-PCR试剂盒从减少的胎盘总细胞RNA量中提取人绒毛膜促性腺激素α（hCGα）的RT-PCR。使用MasterPure™CompleteDNA和RNA纯化试剂盒分离RNA。按照提供的方案，使用递减的总RNA量进行40个循环的扩增。M道，100bp梯形图。Figure2.RT-PCRamplificationofhumanchorionicgonadotropinalpha(hCGα)fromdecreasingamountsofplacentaltotalcellularRNAusingMasterAmp™RT-PCRKitforHighSensitivity.RNAwasisolatedusingtheMasterPure™CompleteDNAandRNAPurificationKit.A40-cycleamplificationwasperformedusingdecreasingamountsoftotalRNAfollowingtheprotocolsupplied.LaneM,100-bpladder.图3.使用用于高灵敏度的MasterAmp™RT-PCR试剂盒或其他制造商的试剂盒，从各种总量的人胎盘RNA中扩增人CGα。严格按照每个制造商的协议进行所有实验。Figure3.HumanCGαwasamplifiedfromvariousamountsoftotalhumanplacentalRNAusingMasterAmp™RT-PCRKitforHighSensitivityorkitsfromothermanufacturers.Allexperimentswerecarriedoutstrictlyaccordingtoeachmanufacturer'sprotocols.用于高灵敏度的MasterAmp™RT-PCR试剂盒。货号尺寸MasterAmp™RT-PCR试剂盒，高灵敏度RT7122525反应RT712100100个反应内容：RetroAmp™RTDNA聚合酶，20XRT-PCR缓冲液，MasterAmp™10XPCR增强剂，25毫摩尔MgCl2，25毫的MnSO4，dNTP混合物各2.5mM，控制模板和引物混合物，无菌水中。CatalogNo.SizeMasterAmp™RT-PCRKitforHighSensitivityRT7122525ReactionsRT712100100ReactionsContents:RetroAmp™RTDNAPolymerase,20XRT-PCRBuffer,MasterAmp™10XPCREnhancer,25mMMgCl2,25mMMnSO4,dNTPMix2.5mMeach,ControlTemplateandPrimerMix,SterileWater.对此产品有兴趣，可联系中北林格获取更多详细信息。</p>)