epicentreAA50125AmpliScribe™T7Aminoallyl-RNATranscriptionKit产品简介：北京中北林格供应epicentreAA50125AmpliScribe™T7Aminoallyl-RNATranscriptionKit，中北林格是epicentre***，提供epicentre产品货号报价查询。其他包括lucigen***，cellscript***，Biosearchtechnologies***，epicentre***，illumina***，ICLLAB***，ImmunoReagents***。</p产品名称:AmpliScribe™T7Aminoallyl-RNATranscriptionKit货号:AA50125规格:25Rxns存储温度:-20℃品牌：lucigen/epicentre</p>供应商：中北林格</p>产地：us</p>发货地：北京</pepicentreAA50125AmpliScribe™T7Aminoallyl-RNATranscriptionKit产品描述AmpliScribe™T7氨基烯丙基RNA转录***盒应用领域用于任何目的的非***性RNA探针的生产，包括原位杂交和印迹实验。ProductionofnonradioactiveRNAprobesforanypurpose,includinginsituhybridizationandblottingexperiments.AmpliScribe™T7氨基烯丙基RNA转录***盒可高产率合成氨基烯丙基标记的RNA（AA-RNA）。该***盒将5-（3-氨基烯丙基）-UTP（AA-UTP）整合到RNA转录物中。由于将AA-UTP以与UTP相同的效率掺入RNA中，所以获得了高产率的AA-RNA。然后可以用胺反应性生物素化***或荧光染料处理AA-RNA产物，分别获得生物素标记的或荧光标记的RNA。已经优化了反应条件，以持续生成均一标记的AA-RNA，该AA-RNA与荧光染料或生物素化合物结合后可产生高信号强度。与在体外将荧光或生物素标记的NTP直接掺入RNA相比，这种非***性标记的RNA的制备方法更有效，更便宜。转录。TheAmpliScribe™T7Aminoallyl-RNATranscriptionKitenableshigh-yieldsynthesisofaminoallyl-labeledRNA(AA-RNA).Thekitincorporates5-(3-aminoallyl)-UTP(AA-UTP)intotheRNAtranscript.SinceAA-UTPisincorporatedintoRNAwiththesameefficiencyasUTP,highyieldsofAA-RNAareobtained.TheAA-RNAproductcanthenbetreatedwithamine-reactivebiotinylationreagentsorfluorescentdyestoobtainbiotin-labeledorfluorescentlylabeledRNA,respectively.Reactionconditi***h***ebeenoptimizedtoc***istentlygenerateuniformlylabeledAA-RNAthat,uponconjugationtoafluorescentdyeorbiotincompound,yieldshighsignalintensity.ThismethodformakingnonradioactivelylabeledRNAismoreefficientandlessexpensivethandirectincorporationoffluorescent-orbiotin-labeledNTPsintoRNAduringinvitrotranscription.注意：建议使用TargetAmp™氨基烯丙基aRNA扩增***盒来制备标记的靶标，以与DNA微阵列杂交。Note:TargetAmp™Aminoallyl-aRNAAmplificationKitsarerecommendedformakinglabeledtargetforhybridizingtoDNAmicroarrays.好处说明该***盒使用EPICENTRE的AmpliScribe高产量体外转录技术来产生***高可能的氨基烯丙基RNA产量。ThekitusesEPICENTRE'sAmpliScribehigh-yieldinvitrotranscriptiontechnologytogeneratethehighestpossibleyieldofaminoallyl-RNA.优化了体外转录反应条件，以产生***佳水平的均匀标记的AA-RNA，以实现染料或生物素结合后的高信号强度。Invitrotranscriptionreactionconditi***areoptimizedtoyieldoptimallevelsofuniformlylabeledAA-RNAforhighsignalintensityafterdyeorbiotinconjugation.Moreefficientandlessexpensivethandirectincorporationoffluorescent-orbiotin-labeledNTP.与直接掺入荧光或生物素标记的NTP相比，效率更高，成本更低。RNaseInhibitorincluded.包括RNase***剂。epicentreAA50125AmpliScribe™T7Aminoallyl-RNATranscriptionKit图例说明表1.AmpliScribe™T7氨基烯丙基RNA转录***盒即使从有限数量的DNA模板中也可产生非常高的氨基烯丙基RNA产量Table1.TheAmpliScribe™T7Aminoallyl-RNATranscriptionKitproducesveryhighyieldsofaminoallyl-RNAevenfromlimitingamountsofDNAtemplate.对此产品有兴趣，可联系中北林格获取更多详细信息。)