epicentreNT0911-100Nextera™DNASamplePrepKit产品简介：北京中北林格供应epicentreNT0911-100Nextera™DNASamplePrepKit，中北林格是epicentre总代理，提供epicentre产品货号报价查询。其他包括lucigen总代理，cellscript总代理，Biosearchtechnologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>产品名称:Nextera™DNASamplePrepKit(RocheTitanium-compatible)货号:NT0911-100规格:100Rxns(2xNT0911-50)存储温度:-20℃品牌：lucigen/epicentre</p>供应商：中北林格</p>产地：us</p>发货地：北京</p产品描述epicentreNT0911-100Nextera™DNASamplePrepKit应用领域制备与IlluminaSolexa®下一代测序仪器兼容的DNA文库。PreparationofDNAlibrariescompatiblewithIlluminaSolexa®next-generationsequencinginstruments.制备与Roche454™GSFLX和GSFLXTitanium™下一代测序仪器兼容的DNA文库。PreparationofDNAlibrariescompatiblewithRoche454™GSFLXandGSFLXTitanium™next-generationsequencinginstruments.Nextera™DNA样品制备试剂盒（与Illumina兼容）专门设计用于制备与IlluminaSolexaGAI和GAII仪器兼容的基因组DNA文库。Nextera技术利用体外转座*在单管反应中同时片段化和标记DNA。与现有方法相比，Nextera文库制备方法具有明显的优势，例如雾化，超声处理或剪切（表1）。TheNextera™DNASamplePrepKit(Illumina-Compatible)isspecificallydesignedtopreparegenomicDNAlibrariescompatiblewithIlluminaSolexaGAIandGAIIinstruments.Nexteratechnologyemploysinvitrotransposition*tosimultaneouslyfragmentandtagDNAinasingle-tubereaction.TheNexteralibrarypreparationmethodofferssignificantadvantagesoverexistingmethods,suchasnebulization,sonication,orshearing(Table1).Nextera™DNA样品制备试剂盒（与Roche454兼容）是专门设计用于制备与Roche454GSFLX和GSFLXTitanium仪器兼容的基因组DNA文库。Nextera技术利用体外转座*在单管反应中同时片段化和标记DNA。与现有方法相比，Nextera文库制备方法具有明显的优势，例如雾化，超声处理或剪切（表1）。Nextera™DNASamplePrepKits(Roche454-Compatible)arespecificallydesignedtopreparegenomicDNAlibrariescompatiblewithRoche454GSFLXandGSFLXTitaniuminstruments.Nexteratechnologyemploysinvitrotransposition*tosimultaneouslyfragmentandtagDNAinasingle-tubereaction.TheNexteralibrarypreparationmethodofferssignificantadvantagesoverexistingmethods,suchasnebulization,sonication,orshearing(Table1).epicentreNT0911-100Nextera™DNASamplePrepKit好处说明仅需要50ng的起始DNA。在不到2小时的时间内准备好可测序的文库。结合特定于平台的标签和可选的条形码（图1）。在IlluminaSolexaGAI和GAII仪器上验证。ValidatedonIlluminaSolexaGAIandGAIIinstruments.在Roche454GSFLX和GSFLXTitanium平台上验证。ValidatedonRoche454GSFLXandGSFLXTitaniumplatforms.epicentreNT0911-100Nextera™DNASamplePrepKit图例说明图1（单击放大）。生成用于IlluminaSolexa®平台的库。将目标DNA片段化，并用Nextera™酶混合物标记，该酶混合物的末端带有测序引物位点（蓝色和橙色）。具有四引物反应的受限循环PCR将桥式PCR（bPCR）兼容适配器（紫色和粉红色）添加到核心测序库中。可以在下游bPCR适配器（粉红色）和核心测序库适配器（橙色）之间添加可选的条形码（三角形）。IlluminaSolexa兼容文库需要其他测序引物：阅读1个引物（蓝色/灰色箭头）；阅读2底漆（橙色/灰色箭头）；和IndexReadPrimer（灰色/橙色箭头）。Figure1(clicktoenlarge).GenerationoflibrariesforIlluminaSolexa®platforms.TargetDNAisfragmentedandtaggedwithNextera™EnzymeMixcontainingendsappendedwithsequencingprimersites(blueandorange).Limited-cyclePCRwithafour-primerreactionaddsbridgePCR(bPCR)compatibleadaptors(purpleandpink)tothecoresequencinglibrary.Optionalbarcodes(triangle)canbeaddedbetweenthedownstreambPCRadaptor(pink)andthecoresequencinglibraryadaptor(orange).AlternativesequencingprimersarerequiredfortheIlluminaSolexa-compatiblelibraries:Read1Primer(blue/grayarrow);Read2Primer(orange/grayarrow);andIndexReadPrimer(gray/orangearrow).图2（单击放大）。生成用于Roche454™平台的库。用Nextera™酶混合物对基因组DNA进行片段化和标记后，进行四引物有限循环PCR，添加了与Roche454兼容的接头序列（蓝色和橙色）。可以在上游乳液PCR（emPCR）适配器（蓝色）和转座子末端（灰色）之间添加可选的条形码（三角形）。Figure1(clicktoenlarge).GenerationoflibrariesforRoche454™platforms.AfterfragmentingandtagginggenomicDNAwithNextera™EnzymeMix,four-primerlimited-cyclePCRisperformedwhichaddsRoche454-compatibleadaptorsequences(blueandorange).Optionalbarcodes(triangle)canbeaddedbetweentheupstreamemulsionPCR(emPCR)adaptor(blue)andthetransposonend(gray).Nextera™DNA样品制备试剂盒（与Illumina兼容）CatalogNo.SizeNextera™DNASamplePrepKit(Illumina-Compatible)*GA091155ReactionsGA09112020ReactionsGA0911-5050ReactionsGA0911-9696ReactionsContents:NexteraEnzymeMix(Illumina-compatible),5XNexteraReactionBuffer(LMW),5XNexteraReactionBuffer(HMW),50XNexteraPrimerCocktail(Illumina-compatible),50XNexteraAdaptor2(Illumina-compatible),200XNexteraRead1Primer,200XNexteraRead2Primer,200XNexteraIndexReadPrimer,NexteraControlDNA,and2XNexteraPCRBuffer.*Note:TheNextera™PCREnzyme(seebelow)mustbepurchasedseparatelywhenusinganyoftheNextera™DNASamplePrepKits.Nextera™PCREnzyme(required)EM09112020ReactionsEM09115050ReactionsEM0911-9696ReactionsNextera™BarCodes(optional)GABC095012BarCodes(Illumina-Compatible)Note:Thebarcodingkitsareoptional.Eachkitcontains12barcodes,andeachbarcodecanbeusedtoprepareupto50bar-codedlibraries.Nextera™DNA样品制备试剂盒（与Roche454兼容）CatalogNo.SizeNextera™DNASamplePrepKit(RocheTitanium-Compatible)NT091155ReactionsNT09112020ReactionsNT0911-5050ReactionsNT0911-9696ReactionsContents:NexteraEnzymeMix(RocheTi-Compatible),5XNexteraReactionBuffer(HMW),50XNexteraPrimerCocktail(RocheTi-Compatible),50XNexteraAdaptor1(RocheTi-Compatible),NexteraControlDNA,and2XNexteraPCRBuffer.*Note:TheNextera™PCREnzyme(seebelow)mustbepurchasedseparatelywhenusinganyoftheNextera™DNASamplePrepKits.Nextera™DNASamplePrepKit(RocheFLX-Compatible)*FL091155ReactionsFL09112020ReactionsContents:NexteraEnzymeMix(RocheFLX-Compatible),5XNexteraReactionBuffer(HMW),50XNexteraPrimerCocktail(RocheFLX-Compatible),50XNexteraAdaptor1(RocheFLX-Compatible),NexteraControlDNA,and2XNexteraPCRBuffer.*Note:TheNextera™PCREnzyme(seebelow)mustbepurchasedseparatelywhenusinganyoftheNextera™DNASamplePrepKits.Nextera™PCREnzyme(required)EM09112020ReactionsEM09115050ReactionsEM0911-9696ReactionsNextera™BarCodes(optional)FLBC095012BarCodes(RocheFLX-Compatible)NTBC095012BarCodes(RocheTitanium-Compatible)Note:Thebarcodingkitsareoptional.Eachkitcontains12barcodes,andeachbarcodecanbeusedtoprepareupto50bar-codedlibraries.对此产品有兴趣，可联系中北林格获取更多详细信息。</p>)