epicentreRF91025MasterAmp™HighFidelityRT-PCRKit产品简介：北京中北林格供应epicentreRF91025MasterAmp™HighFidelityRT-PCRKit，中北林格是epicentre***，提供epicentre产品货号报价查询。其他包括lucigen***，cellscript***，Biosearchtechnologies***，epicentre***，illumina***，ICLLAB***，ImmunoReagents***。</p>产品名称:MasterAmp™HighFidelityRT-PCRKit货号:RF91025规格:25Rxns存储温度:-20℃品牌：lucigen/epicentre</p>供应商：中北林格</p>产地：us</p>发货地：北京</p>epicentreRF91025MasterAmp™HighFidelityRT-PCRKit产品描述Applicati***HighlyaccurateRT-PCRamplificationofRNAtemplatesforcloning,sequencing,expression,andtranscriptionanalysis.All-purposeRT-PCRinwhichfull-lengthamplificationandhighaccuracyareessential.MultiplexRT-PCR.RNA模板的高精度RT-PCR扩增，可用于***，测序，表达和转录分析。通用RT-PCR，其中全长扩增和高精度至关重要。多重RT-PCR。TheMasterAmp™HighFidelityRT-PCRKitisidealforsynthesisofaccuratedouble-strandedcDNAproductsthatwillbeusedforcloning,sequencing,expression,ortranscriptionanalysis.ThekitusesMMLV-RTPlus,areversetranscriptasewithanunsurpassedaccuracyandreliability,andMasterAmpTAQurate™DNAPolymerase,ahigh-fidelityPCRenzymemix(Fig.1).Thekitalsoincludesanoptimized2XRT-PCRBufferPreMix(containingdNTPs,MgCl2,andbuffer),randomandoligo(dT)primers,andtheMasterAmpPCREnhancerwithbetaine,*whichcansubstantiallyimprovetheyieldandspecificityofRT-PCRamplification.MasterAmp™高保真RT-PCR***盒非常适合合成***的双链cDNA产品，该产品将用于***，测序，表达或转录分析。该***盒使用MMLV-RTPlus（一种具有***的准确性和可靠性的逆转录酶）和MasterAmpTAQurate™DNA聚合酶（一种高保真度PCR酶混合物）（图1）。该***盒还包括优化的2XRT-PCR缓冲液PreMix（包含dNTP，MgCl2和缓冲液），随机和oligo（dT）引物，以及具有甜菜碱的MasterAmpPCR增强剂*，可大大提高RT的产量和特异性。-PCR扩增。BenefitsHigh-fidelityRT-PCR(i.e.,lowerrorrates).Amplificationoffull-lengthproductsfromRNAtemplatesupto~6kb(Fig.2).Randomandoligo(dT)primersincludedforfirst-strandcDNAsynthesis.UniquePreMixreducespipettingstepsandchancesofcontamination.UsesMasterAmpPCREnhancerforbetteramplificationofhigh-GCorotherdifficulttemplates.高保真RT-PCR（即低错误率）。从RNA模板扩增全长产物，***大约6kb（图2）。包括随机和oligo（dT）引物，用于***链cDNA合成。独特的PreMix减少了移液步骤和污染机会。使用MasterAmpPCR增强剂可更好地扩***GC或其他困难的模板。epicentreRF91025MasterAmp™HighFidelityRT-PCRKit图例说明Figure1.DifferentRT-PCRprotocolsthatcanbeusedwiththeMasterAmp™HighFidelityRT-PCRKit.图1.可以与MasterAmp™高保真RT-PCR***盒一起使用的不同RT-PCR方案。Figure2.TheMasterAmp™HighFidelityRT-PCRKitamplifiesfull-lengthproductsfromRNAtemplatesupto~6kb.RT-PCRproductsfromTobaccoMosaicVirusRNAwithprimersthatamplifya5.5-kbregionoftheviralRNA.LaneM,Kilobaseladder;lane1,RT-PCRusingtheMasterAmpone-stepprotocol;lane2,RT-PCRusingMMLV-RTandTaqDNApolymerasewithastandardtwo-stepprocedure;lane3,RT-PCRusingTthDNApolymerase.图2.MasterAmp™高保真RT-PCR***盒可扩增RNA模板中的全长产物，***大约6kb。***花叶病毒RNA的RT-PCR产物，带有引物，可扩增病毒RNA的5.5-kb区域。M线，千磅梯；泳道1，使用MasterAmp一步协议进行RT-PCR；泳道2，使用MMLV-RT和TaqDNA聚合酶的RT-PCR，采用标准的两步操作；泳道3，使用TthDNA聚合酶的RT-PCR。Figure3.AmplificationofavarietyofRNAtemplatesusingaone-stepprotocolwiththeMasterAmp™HighFidelityRT-PCRKit.LaneM,100-bpladder;lane1,humanlungβ-actin;lane2,humanlungGAPDH;lane3,regionofTMV;lane4,E.coli16SrRNA;lane5,humanCGα;lane6,humanheartβ-actin;lane7,regionofTMV.图3.使用MasterAmp™高保真RT-PCR***盒一步一步扩增各种RNA模板。M道，100bp阶梯;泳道1，人肺β-肌动蛋白；泳道2，人肺GAPDH；TMV区域3泳道；泳道4，大肠***16SrRNA；人CGα5泳道；泳道6，人的心脏β-肌动蛋白；TMV的第7车道。Coveredbyissuedand/orpendingpatents.MasterAmp™HighFidelityRT-PCRKit.Purchaseofthisproductincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser’sowninternalresearch.Nootherpatentrights(suchas5´NucleaseProcesspatentrights)areconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.CatalogNo.SizeMasterAmp™HighFidelityRT-PCRKitRF9102525Reacti***RF910100100Reacti***Contents:MMLV-RTPlus,MasterAmp™TAQurate™DNAPolymeraseMix,MasterAmp™2XRT-PCRPreMix(includesdNTPs),MasterAmp™10XRT-PCREnhancer,RandomNonamerPrimer,Oligo(dT)18Primer,ControlTemplateandPrimerMix,SterileWater.对此产品有兴趣，可联系中北林格获取更多详细信息。</p>)